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Showing: 1041 - 1060 of 3431 results

  1. pAN1646

    Plasmid
    #127205
    Purpose
    pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS
    Depositor
    Hana El-Samad
    Article
    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    p7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    127205-plasmid-map-sequence-id-250026

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  5. DRD5-Tango

    Plasmid
    #66272
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    DRD5 (DRD5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66272-plasmid-map-sequence-id-305774

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  10. pMXs-hs-3xHA-7CtoA-LIN-41

    Plasmid
    #52726
    Purpose
    retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations of all 7 cysteines in the RING domain and 3 N-terminal HA tags
    Depositor
    Shinya Yamanaka
    Article
    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.
    Insert
    LIN-41 (TRIM71 Human)
    Use
    Retroviral
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    changed cysteines 12, 15, 61, 66, 69, 91, and 94 …
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    52726-plasmid-map-sequence-id-82095

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  11. pLKO_HSV.NWS.Ollas_mCherry-NLS

    Plasmid
    #178272
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HSV.NWS.Ollas and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178272-plasmid-map-sequence-id-353132

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Showing: 1041 - 1060 of 3431 results