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Plasmid#69334PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2ndmChO intron; use C-term after STOPDepositorInsertrSL2-NLS-mChOint-FgF
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-fishRab8B[Q67L]
Plasmid#24908DepositorInsertRab8B (rab8b Zebrafish)
UseGateway entry vectorTagsExpressionMutationChanged Glutamine 67 to LeucinePromoterAvailable sinceJune 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV4.51-mut
Plasmid#201060PurposeExpresses AAV2 Rep and AAV4.51 CapDepositorInsertAAV4.51 Cap
UseAAVTagsExpressionMammalianMutationN586R, S587V, N588D, L589RPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV4.52-mut
Plasmid#201061PurposeExpresses AAV2 Rep and AAV4.52 CapDepositorInsertAAV4.52 Cap
UseAAVTagsExpressionMammalianMutationN586A, S587G, N588F, L589RPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV4.18-mut
Plasmid#201062PurposeExpresses AAV2 Rep and AAV4.18 CapDepositorInsertAAV4.18 Cap
UseAAVTagsExpressionMammalianMutationK492E, K503E, N585SPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV4.68-mut
Plasmid#201064PurposeExpresses AAV2 Rep and AAV4.68 CapDepositorInsertAAV4.68 Cap
UseAAVTagsExpressionMammalianMutationT591V, D593R, R594G, T596RPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV4.69-mut
Plasmid#201065PurposeExpresses AAV2 Rep and AAV4.69 CapDepositorInsertAAV4.69 Cap
UseAAVTagsExpressionMammalianMutationT591P, D593M, R594G, T596GPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET_SynPspA
Plasmid#223812PurposeBacterial expression of SynPspA with N-terminal 10xHis-tag and EK cleavage siteDepositorInsertSynPspA
UseTags10xHis and NusAExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
S111
Plasmid#174549PurposeConstitutive PhCMV-driven mammalian expression vector of CDH-1-TF cassette (PhCMV-Gal4-Bcl-XL-P2A-LD3-Rel65-pA)DepositorInsertBclxL and designed protein LD3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
S112
Plasmid#174550PurposeConstitutive PhCMV-driven mammalian expression vector of CDH-2-TF cassette (PhCMV-Gal4-Bcl2-P2A-LD3-Rel65-pA)DepositorInsertBcl2 and designed protein LD3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
AP-VAMP7(low)
Plasmid#167998Purposepositive selection PACE Accessory PlasmidDepositorInsertVAMP7(K121-R180)-T7PAP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-CV-VAMP1
Plasmid#168022PurposeExpresses eCFP-VAMP1(T29-K89)-mVenus in bacterial cellsDepositorInserteCFP-VAMP1(T29-K89)-mVenus
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-CV-VAMP4
Plasmid#168025PurposeExpresses eCFP-VAMP4(P48-R107)-mVenus in bacterial cellsDepositorInserteCFP-VAMP4(P48-R107)-mVenus
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-CV-VAMP3
Plasmid#168024PurposeExpresses eCFP-VAMP3(S11-R69)-mVenus in bacterial cellsDepositorInserteCFP-VAMP3(S11-R69)-mVenus
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-CV-VAMP7
Plasmid#168027PurposeExpresses eCFP-VAMP7(K121-R180)-mVenus in bacterial cellsDepositorInserteCFP-VAMP7(K121-R180)-mVenus
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-CV-VAMP5
Plasmid#168026PurposeExpresses eCFP-VAMP5(M1-Q60)-mVenus in bacterial cellsDepositorInserteCFP-VAMP5(M1-Q60)-mVenus
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET_4E1H.95
Plasmid#155213PurposeExpresses 4E1H.95 de novo protein in E.coliDepositorInsert4E1H.95
UseTags6x His TagExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET_4H.01
Plasmid#155210PurposeExpresses 4H.01 de novo designed protein in E.coliDepositorInsert4H.01
UseTags6x His TagExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
4E1H.95_LUMABS
Plasmid#155208PurposeExpresses 4E1H.95_LUMABS sensor in E.coliDepositorInsert4E1H.95_LUMABS
UseTags6x His TagExpressionMutationPromoterAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET_3E2H.37
Plasmid#155211PurposeExpresses 3E2H.37 de novo designed protein in E.coliDepositorInsert3E2H.37
UseTags6x His TagExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET_3H1L_02.395
Plasmid#155198PurposeExpresses 3H1L_02.395 in E.coliDepositorInsert3H1L_02.395
UseTags6x His TagExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIs8-4b-SaDAH::G226D
Plasmid#140130PurposeExpresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase G226D variant from Sinomenium acutum
UseTags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialMutationchanged Gly226 to Asp226PromoterT7Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCx35CT-S276A, S298ter
Plasmid#42914DepositorInsertConnexin 35 C-terminal domain
UseTags6x His, GST, and S-tagExpressionBacterialMutationSer276 to Ala and Ser298 stop codonPromoterT7Available sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
PCx35-pcDNA-S110A, S276A
Plasmid#42926DepositorInsertConnexin 35
UseTagsExpressionMammalianMutationSer110 to Ala and Ser276 to AlaPromoterCMVAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
PCx35-pcDNA-S110A, S276A, S298ter
Plasmid#42927DepositorInsertConnexin 35
UseTagsExpressionMammalianMutationSer110 to Ala, Ser276 to Ala, and Ser298 to stop …PromoterCMVAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT FUCCI
Plasmid#136935Purposedonor plasmid for cardiac-specific FUCCI expression in human cellsDepositorInsertTroponin T promoter
UseSynthetic BiologyTagsExpressionMammalianMutationPromotercTnTAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pAV116
Plasmid#63183PurposepRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-mTagBFP2-MRTF-A
Plasmid#182061PurposeMammalian expression vector encoding myocardin-related transcription factor A tagged with mTagBFP2.DepositorInsertmTagBFP2-MRTF-A (MRTFA Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only