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pLKO-cfNHERF2_5

Plasmid

#195228

Purpose

shRNA to canine NHERF2.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

NHERF2 (SLC9A3R1 canine)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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p8391 LentiCRISPRv2 Neo sgPTPN14-1

Plasmid

#221651

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-1 (PTPN14 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8392 LentiCRISPRv2 Neo sgPTPN14-3

Plasmid

#221652

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-3 (PTPN14 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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BoxB-MS2 adRNA (RAB7A-GAC site)

Plasmid

#170149

Purpose

AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

BoxB-RAB7A(GAC)-MS2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (RAB7A-GAC site)

Plasmid

#170140

Purpose

AAV vector carrying a MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-RAB7A(GAC)-MS2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCdh10.1 GFP

Plasmid

#209102

Purpose

GFP expressing scramble of shRNA targeting Cdh10

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh10.1

Use

Tags

Expression

Mutation

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCdh11.1 GFP

Plasmid

#209103

Purpose

GFP expressing shRNA targeting Cdh11

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCdh11.1

Use

Tags

Expression

Mutation

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCdh2.1 GFP

Plasmid

#209099

Purpose

GFP expressing shRNA targeting Cdh2

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCdh2.1

Use

Tags

Expression

Mutation

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCdh11.1 GFP

Plasmid

#209104

Purpose

GFP expressing scramble of shRNA targeting Cdh11

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh11.1

Use

Tags

Expression

Mutation

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCtnnd2.2-GFP

Plasmid

#209098

Purpose

GFP expressing scramble of shRNA targeting Ctnnd2 3' UTR

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCtnnd2.2

Use

Tags

Expression

Mutation

Promoter

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TST-EGFP-GPIDAF

Plasmid

#213708

Purpose

For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an hPGK promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Use

Tags

Twin-strep-tag

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TST-EGFP-GPIBY55

Plasmid

#213707

Purpose

For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an hPGK promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Use

Tags

Twin-strep-tag

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

Plasmid

#210736

Purpose

Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

SaSp D10A Cas9
Merry sgCAG

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Mutation

D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

Promoter

H1 and eCMV

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

Plasmid

#210735

Purpose

Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

SaSp D10A Cas9
Gollum sgCAG

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Mutation

D10A, N terminal Sa Cas9, C-terminal Sp Cas9

Promoter

H1 and eCMV

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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hLig4 guide2 in pX458

Plasmid

#211536

Purpose

sgRNA-2 against Lig4

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA hLig4 (LIG4 Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

u6

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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hLig4 guide1 in pX458

Plasmid

#211535

Purpose

sgRNA-1 against Lig4

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA hLig4 (LIG4 Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

u6

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAf-CRISPR-ctfR1

Plasmid

#207992

Purpose

CRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

Depositor

Perng-Kuang Chang

Article

Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

Insert

ctfR1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Aspergillus flavus U6

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pACBE-ASR (pXK-607)

Plasmid

#208822

Purpose

Universal Antibiotic Screening Reporter for adenine and cytosine base editors. Key Construct: CMV-PuroR*(ATAACG)-eGFP-hU6-gRNA.

Depositor

Kun Xu

Article

Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

Type

Empty backbone

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV, U6

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pACBE-FSR (pXK-1648)

Plasmid

#208821

Purpose

Universal Fluorescent Surrogate Reporter for adenine and cytosine base editors. Key Construct: CMV-mCherry-CMV-eGFP* (ATAACG)-hU6-gRNA.

Depositor

Kun Xu

Article

Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

Type

Empty backbone

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV, U6

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCtnnd2.1-GFP

Plasmid

#209095

Purpose

GFP expressing shRNA targeting Ctnnd2 N-terminus

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCtnnd2.1

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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