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NYESO alpha into TRAC HDRT Source (pTR 223)

Plasmid

#112023

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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NYESO beta into TRBC1 HDRT Source (pTR 277)

Plasmid

#112024

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-beta

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO beta into TRBC1 HDRT

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pHis-parallel1-NLSH2BCas9

Plasmid

#112065

Purpose

Expression and purification of NLSH2BCas9 for fungal gene editing

Depositor

Jeff Coleman

Article

Wang et al Fungal Genet Biol. 2018 May 12. pii: S10

Insert

NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger

Use

CRISPR

Tags

His tag

Expression

Bacterial

Mutation

Cas9 was codon optimized for Aspergillus niger

Promoter

promoter in vector

Availability

Academic Institutions and Nonprofits only

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pORFE0001

Plasmid

#112070

Purpose

AtCas9 module CDS1

Depositor

Christian Chevalier

Insert

AtCAS9

Use

CRISPR

Tags

Expression

Plant

Mutation

Plant codon optimized Cas9 S. pyogenes

Promoter

Availability

Academic Institutions and Nonprofits only

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pORFE0002

Plasmid

#112071

Purpose

AtCAS9 nickase D10A module CDS1

Depositor

Christian Chevalier

Insert

AtCAS9 nickase D10A

Use

CRISPR

Tags

Expression

Plant

Mutation

Plant codon optimized nCas9 D10A S. pyogenes

Promoter

Availability

Academic Institutions and Nonprofits only

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pORFE0003

Plasmid

#112072

Purpose

AtDeadCAS9 D10A H840A module CDS1

Depositor

Christian Chevalier

Insert

AtDeadCAS9 D10A H840A

Use

CRISPR

Tags

Expression

Plant

Mutation

Plant codon optimized dCas9 D10A S. pyogenes

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pORFE0005

Plasmid

#112073

Purpose

AtDeadCAS9 D10A H840A without stop codon module CDS1ns

Depositor

Christian Chevalier

Insert

AtDeadCAS9 D10A H840A without stop codon

Use

CRISPR

Tags

Expression

Plant

Mutation

Plant codon optimized dCas9 D10A S. pyogenes

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pORFE0014

Plasmid

#112076

Purpose

AtCAS9 nickase D10A module CDS1ns

Depositor

Christian Chevalier

Insert

AtCAS9 D10A without stop codon

Use

CRISPR

Tags

Expression

Plant

Mutation

Plant codon optimized dCas9 D10A S. pyogenes

Promoter

Availability

Academic Institutions and Nonprofits only

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pORFE1052

Plasmid

#112078

Purpose

Overexpression of the AtCAS9 nickase in plant under 2x35S promoter

Depositor

Christian Chevalier

Insert

AtCAS9 D10A

Use

CRISPR

Tags

Expression

Plant

Mutation

Plant codon optimized nCas9 D10A S. pyogenes

Promoter

2x35S

Availability

Academic Institutions and Nonprofits only

Enlarge
pORFE1001

Plasmid

#112079

Purpose

Overexpression of the AtCAS9 in plant under 2x35S promoter

Depositor

Christian Chevalier

Insert

AtCAS9

Use

CRISPR

Tags

Expression

Plant

Mutation

Plant codon optimized Cas9 S. pyogenes

Promoter

2x35S

Availability

Academic Institutions and Nonprofits only

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pCMV_AncBE4max

Plasmid

#112094

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

AncBE4max

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV_ABEmax

Plasmid

#112095

Purpose

A:T-to-G:C base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

ABEmax

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_BE4max_3xHA

Plasmid

#112096

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

BE4max_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_AncBE4max_3xHA

Plasmid

#112097

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

AncBE4max_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_ABEmax_3xHA

Plasmid

#112098

Purpose

A:T-to-G:C base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

ABEmax_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_BE4max_P2A_GFP

Plasmid

#112099

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

BE4max_P2A_GFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_AncBE4max_P2A_GFP

Plasmid

#112100

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

AncBE4max_P2A_GFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_ABEmax_P2A_GFP

Plasmid

#112101

Purpose

A:T-to-G:C base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

ABEmax_P2A_GFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL1 sgRNA(MS2)-MS2-AIDmono

Plasmid

#112127

Purpose

empty sgRNA cloning vector with MS2-AIDmono

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

AID mono mutant (AICDA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

N- mutated and C- terminal truncated, H130A/R131E…

Promoter

hU6,CBh

Availability

Academic Institutions and Nonprofits only

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pLL2 sgRNA(MS2)-MS2-AID3c

Plasmid

#112128

Purpose

empty sgRNA cloning vector with MS2-AID3c

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

AID mono 3c mutant (AICDA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

N- mutated and C- terminal truncated, F115Y/C116F…

Promoter

hU6,CBh

Availability

Academic Institutions and Nonprofits only

Enlarge