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STK36 gRNA (BRDN0001148669)

Plasmid

#77277

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK36

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK36 (STK36 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ROR1 gRNA (BRDN0001162245)

Plasmid

#76043

Purpose

3rd generation lentiviral gRNA plasmid targeting human ROR1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ROR1 (ROR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-VQRRA-P2A-GFP-PGK-Puro

Plasmid

#110864

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mRIPK3 D143N-Flag/cHA-pTRIPZ vector

Plasmid

#73703

Purpose

lentiviral expression of mRIPK3 mutant

Depositor

Francis Chan

Insert

RIPK3 (Ripk3 Mouse)

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

D143N

Promoter

CMV/tet

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only
V2-MESA-35F-Tev-MS2-p65-HSF1

Plasmid

#84513

Purpose

MESA protease chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, and Tev protease, MS2-P65-HSF1

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Inserts

V2-MESA-35F-Tev
MS2-P65-HSF1

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001148995)

Plasmid

#80181

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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DYRK1B gRNA (BRDN0001149482)

Plasmid

#76623

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK1B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK1B (DYRK1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PDGFRB gRNA (BRDN0001147116)

Plasmid

#77060

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDGFRB

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDGFRB (PDGFRB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PDGFRB gRNA (BRDN0001146738)

Plasmid

#77062

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDGFRB

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDGFRB (PDGFRB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKCB gRNA (BRDN0001148507)

Plasmid

#75954

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCB

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCB (PRKCB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKCB gRNA (BRDN0001144990)

Plasmid

#75955

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCB

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCB (PRKCB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shId2 #1

Plasmid

#83088

Purpose

Lentiviral shRNA vector for inducible knockdown of mouse Id2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shId2 (Id2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
JAK3 gRNA (BRDN0001145049)

Plasmid

#77190

Purpose

3rd generation lentiviral gRNA plasmid targeting human JAK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

JAK3 (JAK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLX-V5-TRAF2 S11A-puro

Plasmid

#44112

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S11A (TRAF2 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Serine 11 to Alanine

Promoter

Available since

July 22, 2013

Availability

Academic Institutions and Nonprofits only
pLenti X1 Zeo/pTER shMDC1 (673-1)

Plasmid

#22193

Depositor

Eric Campeau

Insert

sh Mediator of DNA damage checkpoint 1

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only

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pLV-shEsr1

Plasmid

#120720

Purpose

Lentiviral vector that expresses GFP and an shRNA targeting Esr1 (in pLL3.7).

Depositor

Amy Lasek

Article

Satta et al Int J Neuropsychopharmacol. 2018 Apr 1;2

Insert

Esr1 shRNA (Esr1 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Mouse U6

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pPKm-243

Plasmid

#90506

Purpose

mOrange and mitochondrial-tagged sfGFP (pSIN-OFP-mitosfGFP)

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mOrange

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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DYRK1B gRNA (BRDN0001149418)

Plasmid

#76621

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK1B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK1B (DYRK1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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ULK2 gRNA (BRDN0001147288)

Plasmid

#77262

Purpose

3rd generation lentiviral gRNA plasmid targeting human ULK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ULK2 (ULK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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5xHRE-MinTK-CRE

Plasmid

#141145

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

5xHRE-MinTK-CRE

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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