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pMXs-puro GFP-p62 L343A

Plasmid

#38279

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed L343A

Promoter

Available since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only
pLJM1 FLCN (R164A) FLAG GFP

Plasmid

#164980

Purpose

Lentiviral vector for FLCN expression in mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

FLCN (FLCN Human)

Use

Lentiviral

Tags

1xFLAG-GFP

Expression

Mammalian

Mutation

R164A

Promoter

UBC

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-hLMX1A-T2A-copGFP

Plasmid

#170444

Purpose

Lentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.

Depositor

Sang-Hun Lee

Article

Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

Insert

human LMX1A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Suz12-F1-GFP

Plasmid

#91881

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Suz12 (Suz12 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
35_pAAV-ProC8-CatCh-GFP-WPRE

Plasmid

#125943

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC8

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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140_pAAV-ProA33-CatCh-GFP-WPRE

Plasmid

#125916

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA33

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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167_pAAV-ProC34-CatCh-GFP-WPRE

Plasmid

#125968

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC34

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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13_pAAV-ProC71-CatCh-GFP-WPRE

Plasmid

#125976

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC71

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX puro TRIM21 C54Y - GFP

Plasmid

#116942

Purpose

Expression of TRIM21 C54Y -GFP fusion

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

TRIM21 C54Y-GFP (TRIM21 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

C54Y

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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FRB-GFP-Gab1(Y628F/Y660F)

Plasmid

#188658

Purpose

FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

GRB associated binding protein 1 (Gab1 Mouse)

Use

Tags

FRB and GFP

Expression

Mammalian

Mutation

Y628F and Y660F

Promoter

CMV

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-LpIA(wild-type)

Plasmid

#61821

Purpose

Encodes a wild-type sequence of LplA and serves as the negative control for resorufin labeling

Depositor

Alice Ting

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

Insert

E. coli lipoic acid ligase

Use

Tags

6XHis, EGFP, and Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-hCD24-T2A-GFP

Plasmid

#205456

Purpose

lentiviral plasmid for expression of human CD24

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD24 (CD24 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPy-CAG-GFP-IRES-Pac

Plasmid

#48759

Purpose

CAG promoter driving GFP-IRES-Puromycin Acetyl Transferase

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

EGFP

Use

Ppy

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-181a-1-PGK-GFP

Plasmid

#11376

Depositor

Chang-Zheng Chen

Article

Chen et al Science. 2004 Jan 2. 303(5654):83-6.

Insert

miR-181a-1

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

Plasmid

#69117

Purpose

Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
shTACC3

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

LL (566-567aa) mutated to AA. Silent mutations i…

Promoter

CMV

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Neo/pTER shEGFP#2 (w22-2)

Plasmid

#17480

Purpose

3rd gen lentiviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #2

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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SIV URA PCP-NLS-GFPEnvy

Plasmid

#189941

Purpose

Single-integration vector for inserting PCP-NLS-GFPEnvy at the URA3 locus in Saccharomyces cerevisiae

Depositor

Tineke Lenstra

Article

Brouwer et al Nat Struct Mol Biol. 2023 May;30(5):692-

Insert

PCP-NLS-GFPEnvy

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAT15415-BEAR-GFP-target-mCherry

Plasmid

#162995

Purpose

plasmid expressing an sgRNA targeting the BEAR-GFP plasmid along with an mCherry marker

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

sgRNA targeting the BEAR-GFP plasmid

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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fck-Jaws-KGC-GFP-ER2

Plasmid

#65012

Purpose

Lentivirus-mediated expression of Jaws under the CaMKII promoter.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

Lentiviral

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP Rab11a S25N pcDNA3.1 Zeo (+)

Plasmid

#206080

Purpose

dominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recycling

Depositor

Annette Dolphin

Article

Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

Insert

RAB11A (RAB11A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S25N

Promoter

CMV

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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