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[pSK497] pLJC5 Flag-Depdc5(AB)

Plasmid

#109341

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant AB

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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DKC1 F1.3 gRNA

Plasmid

#90652

Purpose

3rd generation lentiviral gRNA plasmid targeting human DKC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DKC1 (Guide Designation F1.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEIAV-iSyn-eGFPW

Plasmid

#80161

Purpose

Expression in mammalian cells

Depositor

Edward Callaway

Article

Cetin et al J Neurophysiol. 2014 May;111(10):2150-9.

Insert

eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

iSyn

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgHAL

Plasmid

#102315

Purpose

genetic depletion of HAL

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgHAL (HAL Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti4/V5-DEST-P85(W583del)

Plasmid

#40229

Depositor

Lynda Chin

Insert

P85(W583del) (PIK3R1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acid 583

Promoter

CMV

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
MAP2K3_pLX307

Plasmid

#98355

Purpose

Lentiviral expression of MAP2K3

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

MAP2K3 (MAP2K3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Q85K

Promoter

E1Fa

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-E542V

Plasmid

#116481

Purpose

Lentiviral expression of PIK3CA E542V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Tags

Expression

Mutation

E542V

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-T599A

Plasmid

#116200

Purpose

Lentiviral expression of BRAF T599A

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

Expression

Mutation

T599A

Promoter

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLCKO_PSMB2_sgRNA_1

Plasmid

#74184

Purpose

lentiviral vector expressing sgRNA targeting PSMB2

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

PSMB2 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO-sh-Myocardin

Plasmid

#100769

Purpose

Lentiviral expression of shRNA targeting MYOCD

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

Lenti-sh-Myocardin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1 EB1 shRNA #1

Plasmid

#37928

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 shRNA #1 (MAPRE1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pCF204

Plasmid

#121656

Purpose

U6-sgRNA EFS-Cas9-wt-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJZC73

Plasmid

#62341

Purpose

sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells

Depositor

Stanley Qi, Wendell Lim

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
COM-KRAB

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

Targets sv40 promoter, sequence: GAATAGCTCAGAGGCC…

Promoter

CMV and U6

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Neo/pTER shEGFP#1 (w21-2)

Plasmid

#17479

Purpose

3rd gen lentiviral eGFP shRNA #1 (H1/TO promoter), 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #1

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLKO-tet-puro-sh-ex16

Plasmid

#35167

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pLenti CMV Hygro DHFR.cHSF1

Plasmid

#134738

Purpose

Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202

Promoter

CMV

Available since

Oct. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC1-Rap2A-3xFLAG

Plasmid

#87972

Purpose

Expression of Rap2A in mammalian cells

Depositor

David Sabatini

Article

Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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RPS6KA4 gRNA (BRDN0001487122)

Plasmid

#77919

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA4 (RPS6KA4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-375-Decoy.hPGK.GFP.WPRE

Plasmid

#46617

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-375 Decoy (MIR375, Mir375 Mouse, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
PIK3CA gRNA (BRDN0001146960)

Plasmid

#77415

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CA (PIK3CA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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