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Plasmid#83156PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S17N mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationS17NPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGW-GFP-GOI-TurboID
Plasmid#209382PurposeGateway compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMC-J6-dCas9-24XGP41-J9
Plasmid#202006PurposeMoClo level 0 dCas9-24XGP41 coding sequence moduleDepositorInsertdCas9-24XGP41
UseSynthetic Biology; Moclo cloning vectorTagsGS linkersExpressionMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pScDD2_ERdd
Plasmid#109047Purposedestabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).DepositorInsertER50 (ESR1 H. sapiens gene with yeast enhanced codon)
UseTagsyeast enhanced GFP with 8 amino acids TSGRGEGQ li…ExpressionYeastMutation3 mutations : E380G, R434G, N532SPromoterSOD1Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
YEp181-CUP1-His-Smt3
Plasmid#99536PurposeYeast episomal vector for expression of His-tagged Smt3 (SUMO); LEU2 markerDepositorInsertSMT3 (SMT3 Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJOG824
Plasmid#105401PurposeSynthetic biologyDepositorInsertSV40 nuclear localization signal
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceMarch 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG_N2N3-HIS-ybbr
Plasmid#101238PurposeSdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertSdrG_N2N3
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMU-AUTr
Plasmid#61204PurposeFluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoterDepositorInsertLC3(ATG8)
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Golgi-ZapCY1
Plasmid#36322DepositorInsertGolgi-ZapCY1 high affinity Zn(II) sensor
UseTagsGalT (first 60 amino acids)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCA_e-2
Plasmid#202097PurposeReceiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL3 GATA Luc
Plasmid#85695PurposeLuciferase reporter containing three repeats of the GATA consensus siteDepositorInsert3xGATA and minimal MT promoter
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoter3xGATA and minimal MT promoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ154
Plasmid#69305PurposeGateway entry vector with AteCas9 (Arabidopsis codon optimized)DepositorInsertAteCas9 (Arabidopsis codon optimized)
UseCRISPRTagsExpressionMutationNLS fusionPromoterAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_7
Plasmid#60578Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_7
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRBAD-link-CGFP
Plasmid#52733PurposeL-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assayDepositorInsertlink-CGFP
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSW039
Plasmid#140035PurposeKO of PCC 11901 psbA2 gene/expresses YFP under control of inducible cLac143 promoterDepositorInsertYellow fluorescent protein
UseTagsExpressionBacterialMutationPromotercLac143Available sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
6CDG (GID4)
Plasmid#119961PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
UseTags6xHis-TEVExpressionBacterialMutationcontains amino acids 124-289PromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSB1C3-H3
Plasmid#109381PurposeArabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coliDepositorInsertpARABAD-VioB-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b G12R
Plasmid#83143PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12R mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12RPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-PRKAA1ΔC
Plasmid#30306DepositorInsertAMP-activated Protein Kinase α1 ΔC (Prkaa1 Rat)
UseTagsGFPExpressionMammalianMutationdeleted amino acids Pro 526 to Gln 548PromoterAvailable sinceAug. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
YIp128-P30-His-POL30
Plasmid#99542PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only