Addgene

We narrowed to 16,189 results for: multi

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pX330A_dCas9-1x2

Plasmid

#63596

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D1361Y

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAM5411

Plasmid

#132663

Purpose

RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Inserts

aadA
YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PaadA and PconII

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomApple-Kan

Plasmid

#44957

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mApple with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomApple

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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SuperNova-N1

Plasmid

#131853

Purpose

Expresses SuperNova with C-terminal Myc-tag and N-terminal multiple cloning site

Depositor

Geert van den Bogaart

Article

Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

Insert

SuperNova

Use

Tags

Myc tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pADH110

Plasmid

#90982

Purpose

NAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Inserts

NAT 2 of 2
pSNR52

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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bZiPro

Plasmid

#86846

Purpose

express NS2B-NS3 protease in E.coli

Depositor

Dahai Luo

Article

Zhang et al Science. 2016 Dec 8. pii: aai9309.

Inserts

NS3 1-177
NS2B45-96

Use

Tags

a hexahistidine tag and a thrombin cleavage site

Expression

Bacterial

Mutation

NS2B cofactor region (residues 45–96) and NS3 pro…

Promoter

T7

Available since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pHBV-S1-Luc

Plasmid

#71416

Purpose

To see S1 promoter activity of HBV for luciferase assay

Depositor

Wang-Shick Ryu

Article

Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

Insert

HBV S1 promoter driving Luciferase expression

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

HBV S1

Available since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pHBV-S2-Luc

Plasmid

#71417

Purpose

To see HBV S2 promoter activity for luciferase assay

Depositor

Wang-Shick Ryu

Article

Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

Insert

HBV S2 promoter driving luciferase expression

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

HBV S2

Available since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Gata4-GFP donor

Plasmid

#113115

Purpose

Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata4 donor region including homology arms and eGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_FliC_IRES_mCherry_PGK_tBFP

Plasmid

#85438

Purpose

Lentiviral vector for Gal4 inducible S. Typhi Flagellin with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

S. typhi FliC

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only
pmec-4::Chrimson::SL2::mCherry::unc-54

Plasmid

#107745

Purpose

Express Chrimson and mCherry in mechanosensory neurons.

Depositor

Andrew Leifer

Article

Liu et al Elife. 2018 Jun 26;7. pii: 36419. doi: 1

Inserts

Chrimson
mCherry

Use

Tags

Expression

Insect and Worm

Mutation

Promoter

mec-4

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT21.RY

Plasmid

#201006

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-mCherry-sYFP2, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

mcherry
sYFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT22.RY

Plasmid

#201018

Purpose

pLv1-pUC18R6KT-miniTn7-Km-mCherry-sYFP2, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

mcherry
sYFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT22.YB

Plasmid

#201019

Purpose

pLv1-pUC18R6KT-miniTn7-Km-sYFP2-TagBFP, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sYFP2
TagBFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-MCS-Ds

Plasmid

#102416

Purpose

Empty vector with a multiple cloning site flanked by Ds integration elements

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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p458 VQR

Plasmid

#101727

Purpose

Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Use

Tags

Expression

Mammalian

Mutation

VQR

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMVS1111A:PhmtB-bgaB

Plasmid

#205998

Purpose

Conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus encoding beta-galactosidase

Depositor

Bastian Molitor

Article

Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

Insert

thermostable beta-galactosidase

Use

Tags

Expression

Mutation

Promoter

PhmtB

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTK295

Plasmid

#59564

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn Kozak EGFP

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPR-S12

Plasmid

#84031

Purpose

To episomally express codon optimized Cas9 and chimeric guide RNA

Depositor

Sen Wu

Article

Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

Inserts

hSpCas9
eGFP

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Promoter

CMV and CMV (downstream of F2A self-cleaving pept…

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTK296

Plasmid

#59565

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP-PEST

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn Kozak EGFP-PEST

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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