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Plasmid#194803PurposeCasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.DepositorInsertCasRX
UseTagsExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-Lifeact
Plasmid#109545PurposeMultisite gateway vector for 5' tagging with the actin binding motif LifeactDepositorInsertactin binding motif Lifeact
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR43.6r
Plasmid#63197PurposeExpresses CcaS, Ho1, and PcyA constitutivelyDepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcNDA-NES-PhoCl2c-mCherry
Plasmid#164037PurposeOptogenetic control of protein localization by PhoCl2cDepositorInsertNES-PhoCl2c-mCherry
UseTagskozak sequenceExpressionMammalianMutationPromoterCMV promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
ULK1 KO sgRNA
Plasmid#207561PurposepX330 expressing Cas9 and a sgRNA targeting the ULK1 locusDepositorInsertCCCGCCTGCGCCATGGAGCC
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSFS2A-CaKan
Plasmid#189565PurposeCodon optomized Kan resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicans using G418 selectionDepositorInsertCaKan
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
p177bp-LacOx50-Hyg
Plasmid#210213PurposeIntegrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.DepositorInsert50xLacO repeats derived from: PMID: 12864855.
UseTagsExpressionMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-OTI-TCRA_p2_TCRB
Plasmid#131033PurposeExpresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptideDepositorArticleUseTagsExpressionMammalianMutationPromoterCMV/T7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM704
Plasmid#68891PurposeIPTG-inducible CRISPRi vector targeting BT1854, pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
UseTagsExpressionMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
5HT6-mCherry-G-GECO1.0
Plasmid#47500PurposeFluorescent reporter for Ca2+ inside primary cilia with mCherry referenceDepositorInsertG-GECO1.0
UseTags5HT6 and mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTOX8
Plasmid#127453PurposeEncodes MqsR toxin and tsPurple chromoprotein. CAM-RDepositorInsertsmqsR toxin
tsPurple chromoprotein
UseTagsExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterapFAB46 and pRhaAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
TS246_pNKI-2-13_HALO-DPN7
Plasmid#200720Purposeproducing Methyl Tracer HALO protein in bacteriaDepositorInsertHALO-DPN7
UseTags6xHisExpressionBacterialMutationcodon optimizedPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB070.iodo.5
Plasmid#99397PurposeExpression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanineDepositorInsertPN25 TetR; Ptet Iodo.5RS; PproK tyrT(Opt,CUA)
UseTagsExpressionBacterialMutationPromoterPN25, Ptet, and PproKAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTK297
Plasmid#59566PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae 4xFF4DepositorInsertSGP(14) Kozak mKate-G8-L7Ae 4xFF4
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMH48
Plasmid#131861PurposeProduces holo-Cph1* in E. coliDepositorInsertsCph1(1-514,Y263F)-RGD-His6-Cys
HO1
PcyA
UseTagsExpressionBacterialMutationPromoterT7 promoter-1 and T7 promoter-2Available sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-pMJ915v2
Plasmid#88916PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
1xNLS-pMJ915v2
Plasmid#88915PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertCas9
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJW1512
Plasmid#62366PurposeN-terminal 3X FLAG fused to wt BirA - mCherry - sec61ßDepositorInsert3xFLAG-BirA-mCherry-Sec61beta tail anchor (SEC61B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterpTRE2Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEGFP-SpHis5
Plasmid#44836PurposeYeast tagging vector to create gene fusion with yeast optimized EGFP with SpHis5 selectionDepositorInsertyoEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNO183
Plasmid#101068PurposeppsR2-bphP1 bicistronic operon plasmidDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationinsert guanine at position 430 to fix frameshift …PromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only