Addgene

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NK649

Plasmid

#109105

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_06009

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3D11

Plasmid

#71007

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Acf1

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pSKB3.TM1777

Plasmid

#11421

Depositor

Sung-Hou Kim

Article

Shin et al Biochemistry. 2003 Nov 25. 42(46):13429-

Insert

NusA (nusA T. maritima)

Use

Tags

6x His and TEV protease site

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pGGEV_5'_Linker

Plasmid

#49291

Purpose

Golden Gate entry vector at position 5, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR221-Phelo

Plasmid

#48995

Purpose

Gateway adapted transfer of Phleomycin resistance gene

Depositor

Rick Tarleton

Article

Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

Insert

Sh ble

Use

Gateway attb1 & attb2

Tags

Expression

Mutation

Promoter

Available since

April 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pYZ182

Plasmid

#98424

Purpose

Pombe Expression Vector - nmt1 promoter with empty BsaI-pad - no marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ189

Plasmid

#98427

Purpose

Pombe General Purpose Vector - with empty BsaI-pad for new potential markers

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET28CLY8

Plasmid

#21767

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 8 SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pGGEV_4'_XcmI-LacZ

Plasmid

#49305

Purpose

Golden Gate entry vector at position 4, LacZ, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4G02

Plasmid

#71025

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG3711

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
NK619

Plasmid

#109089

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mCherry

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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NK611

Plasmid

#109088

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mCherry

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NK610

Plasmid

#109087

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mCherry

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTLNXA7

Plasmid

#49033

Purpose

FX cloning Xenopus oocyte expression vector with SP6 promoter and N-terminal chicken alpha7 signal sequence

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Xenopus oocytes expression

Tags

chicken alpha7 signal sequence

Expression

Mutation

Promoter

SP6

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF1G06

Plasmid

#70815

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG10133

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pGGEV_3'_Linker

Plasmid

#49289

Purpose

Golden Gate entry vector at position 3, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG/HA-hBIG1

Plasmid

#226253

Purpose

Expresses N-terminally HA-tagged hBIG1 in mammalian cells

Depositor

Hye-Won Shin

Insert

BIG1 (ARFGEF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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E2_pENTR_R4-R3_HSPter-RPS5A-NLS

Plasmid

#193371

Purpose

nTALEN or nTALECD vector assembly, Entry clone for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR1a-jAspSnFR3

Plasmid

#203460

Purpose

jAspSnFR3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

Plasmid

#132927

Purpose

pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Insert

humanized SpCas9

Use

CRISPR and Synthetic Biology; Pmagic gateway entr…

Tags

Expression

Mutation

Promoter

Available since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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