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Plasmid#99229PurposeMammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.DepositorInsertpr-mEos2
UseTagsLifeactExpressionMammalianMutationA69T for primed conversion applicationsPromoterCMVAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2/CMV-eGFP.PB/PTK
Plasmid#170540PurposeExpresses the puromycin resistance gene fused to a thymidine kinase, upon excision of this cassette the reading frame of eGFP is restoredDepositorInsertDelta 5'-eGFP CMV-PuroTK- Delta 3'-eGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBT/EFS-Cas9-PuroTk
Plasmid#170542PurposeExpresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinaseDepositorInsertPuroTK
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE
Plasmid#140228PurposeRRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.DepositorInsertdLbCpf1 (Cas12a) N terminal half (406) with zipper
UseLentiviralTagsBFPExpressionMammalianMutationPromoterRRM2 endogenous promoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF4 CMV 5TOP intron renilla TRICK CTE polyA
Plasmid#64544Purpose5′ TOP TRICK reporter mRNADepositorInsert12x PP7 stem-loops
UseTags24xMS2 stem loops, 5' TOP (5′ terminal oligo…ExpressionMammalianMutationPromoterTetracycline-inducible promoter and 5′ UTR of hum…Available sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEA01
Plasmid#170766PurposeUtilisation of pSAM2 excision tool in Amycolatopsis sppDepositorInsertsxis, int
oriT
pac
UseTagsExpressionBacterialMutationPromotertrcpAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
SFFV-AID-CTERM-GFP
Plasmid#185768PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, AIDExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSynapsin_NLS_GFP_P2A_SNAP_NR1
Plasmid#170458PurposeExpresses SNAP tagged NR1 in neuronal cellsDepositorInsertNR1 (Grin1 Rat)
UseLentiviralTagsSNAPExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-NeoR_dCas9-Sniper-KRAB_sgSTK3i_#2 (NeoR)
Plasmid#209775PurposeCRISPRi for STK3DepositorInsertdCas9-Sniper-KRAB, sgSTK3i (STK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV.5’-LMNA-eGFP-KI
Plasmid#170545PurposeExpresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNADepositorInsertsLeft Homology arm for LMNA knockin
Right Homology arm for VIM knockin
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV.5’VIM-eGFP-KI
Plasmid#170547PurposeExpresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIMDepositorInsertsLeft Homology arm for VIM knockin
Right Homology arm for VIM knockin
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-LpIA(wild-type)
Plasmid#61821PurposeEncodes a wild-type sequence of LplA and serves as the negative control for resorufin labelingDepositorInsertE. coli lipoic acid ligase
UseTags6XHis, EGFP, and FlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W
Plasmid#163169PurposeLentiviral plasmid with non-targeting gRNA, co-expression of BFP tagDepositorInsertNon-targeting
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tet-myr-CSK-GFP
Plasmid#83470PurposeLentiviral vector for doxycycline-inducible expression of membrane targeted-CSK in mammalian cellsDepositorInsertCSK (CSK Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterdoxycycline-inducible promoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CCNB1-P2A-Hygro_Barcode
Plasmid#170218PurposeBarcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCCNB1 (CCNB1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mKikGR1 (mKikGR1-V69T)
Plasmid#99227PurposeBacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).DepositorInsertpr-mKikGR1
UseTagsExpressionBacterialMutationmonomeric variant with mutation V69T (Eos notatio…PromoterT7Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode
Plasmid#170248PurposeBarcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertPTEN (PTEN Human)
UseLentiviralTagsExpressionMutationC124SPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-LplA(AAG)
Plasmid#61307PurposeContains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expressionDepositorInsertE. coli lipoic acid ligase
UseTags6xHis, Flag, T7 tag, and Xpress epitopeExpressionMammalianMutationE20A, F147A, H149GPromoterCMVAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Actin-C-18
Plasmid#53978PurposeLocalization: Actin, Excitation: 487, Emission: 509DepositorInsertActin (ACTB Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
eGFP-KCC2
Plasmid#104075Purposeexpresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2DepositorInsertKCC2 (b isoform) (Slc12a5 Rat)
UseTagsEGFPExpressionMammalianMutationEcoR1 site in rat KCC2 was removed by silent muta…PromoterCMVAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only