Showing: 1021 - 1040 of 2422 results
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Plasmid#202824PurposeLentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expressionDepositorInserttwo sgRNAs (each with a U6 promoter) to delete SVA within CDK5RAP2 gene
UseLentiviralTagsExpressionMammalianMutationPromoterU6 - twoAvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-sgRNAs-del-CHAF1B-SVA-BFP
Plasmid#202825PurposeLentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CHAF1B gene with EF-1apha for Cas9 and BFP expressionDepositorInserttwo sgRNAs (each with a U6 promoter) to delete intronic SVA within CHAF1B gene
UseLentiviralTagsExpressionMammalianMutationPromoterU6 - twoAvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-sgRNAs-del-SCN8A-SVA-BFP
Plasmid#202826PurposeLentiviral vector modified to express two sgRNAs that delete the intronic SVA within the SCN8A gene with EF-1apha for Cas9 and BFP expressionDepositorInserttwo sgRNAs (each with a U6 promoter) to delete intronic SVA within SCN8A gene
UseLentiviralTagsExpressionMammalianMutationPromoterU6 - twoAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2
Plasmid#205160Purposeexpress β-ACTIN-3′UTR-8xMS2DepositorInsertβ-ACTIN-3′UTR-24xMS2
UseTagstagBFPExpressionBacterial and MammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2
Plasmid#205161Purposeexpress β-ACTIN-3′UTR-24xMS2DepositorInsertβ-ACTIN-3′UTR-24xMS2
UseTagstagBFPExpressionBacterial and MammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1
Plasmid#214995PurposeExpress the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.DepositorInsertEEA1 (EEA1 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro
Plasmid#211462PurposeLentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLIDepositorInsertApaLI
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterTREAvailabilityAcademic Institutions and Nonprofits only -
TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro
Plasmid#211463PurposeLentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)DepositorInsertApaLI-dead
UseLentiviralTagsFLAGExpressionMammalianMutationD211A, E225A, K227APromoterTREAvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(RUNX1(13))-hU6gRNA5(RUNX3(12))-PGKpuroBFP-W
Plasmid#208569PurposeLentiviral vector expressing gRNA targeting human RUNX1 and RUNX3DepositorUseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(RUNX1(13))-hU6gRNA5(RUNX3(13))-PGKpuroBFP-W
Plasmid#208570PurposeLentiviral vector expressing gRNA targeting human RUNX1 and RUNX3DepositorUseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcg 2.0-BFP-circ-arRNA backbone
Plasmid#213965Purposepcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences oDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2- GABARAP
Plasmid#212106PurposeExpression of GABARAP split tandem protein with mTagBFP2DepositorInsertGABARAP(G116A)-mTagBFP2-GABARAP (GABARAP Human, Entacmaea quadricolor)
UseTagsmTagBFP2ExpressionMammalianMutationG116A (lipidation deficiency) of upstream GABARAPPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP
Plasmid#212107PurposeExpression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23ADepositorInsertGABARAP(5X/G116A)-mTagBFP2-GABARAP (GABARAP Human, Entacmaea quadricolor)
UseTagsmTagBFP2ExpressionMammalianMutation5X: K2A, K13A, R15A, K20A, K23A mutations of upst…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO mTagBFP2-GABARAP(G116A)-GABARAP
Plasmid#212109PurposeExpression of N-terminally tagged GABARAP tandem construct with mTagBFP2DepositorInsertmTagBFP2-GABARAP(G116A)-GABARAP (GABARAP Human, Entacmaea quadricolor)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1
Plasmid#198240PurposeFor I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sitesDepositorInsertsUseZebrafish transgenesisTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
lenti-UCOE-dCas9-BFP-Zim3-KRAB
Plasmid#196716PurposeCRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3DepositorInsertdCas9-TagBFP-KRAB(ZIM3)
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationD10A, N863APromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pCW-Tet-Cas9-BFP-PGK-Blast
Plasmid#196720PurposeTet-inducible expression of Cas9-T2A-TagBFPDepositorInsertCas9-T2A-TagBFP
UseCRISPR and LentiviralTags3xFLAG-SV40NLS and Nucleoplasmin NLSExpressionMutationPromoterTRE, PGKAvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(AAVS1)-hU6gRNA5(BbsI)-PGKpuroBFP-W
Plasmid#200502PurposeLentiviral vector expressing gRNA targeting human AAVS1DepositorInsertAAVS1 (AAVS1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(AAVS1)-PGKpuroBFP-W
Plasmid#200503PurposeLentiviral vector expressing gRNA targeting human SMARCA4 and AAVS1DepositorUseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(46))-PGKpuroBFP-W
Plasmid#200504PurposeLentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2DepositorUseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1021 - 1040 of 2422 results