1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pICH47742:FCP:Cas9YFP

   Plasmid

   #85986

   Purpose

   Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Insert

   Cas9
   

   Use

   CRISPR; Golden gate assembly

   Tags

   SV40 NLS, YFP

   Expression

   Mutation

   Promoter

   FCP

   Available since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-PxCas12a-2AeGFP

   Plasmid

   #123637

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   PxCas12a
   

   Use

   Tags

   3xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pZmUBQ:Mla1

   Plasmid

   #132355

   Purpose

   Expression of the barley Mla1 NLR from the Zea mays ubiquitin promotor

   Depositor

   Paul Schulze-Lefert

   Article

   Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

   Insert

   HvMla1
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pZmUBQ

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEU3-NII-FLAGLICNot

   Plasmid

   #140184

   Purpose

   Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, FLAG-tag and NotI restriction site

   Depositor

   Tamás Mészáros

   Article

   Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

   Type

   Empty backbone

   Use

   Expression vector for a cell-free system

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   T7

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLck-mVenus-C1

   Plasmid

   #84337

   Purpose

   Expresses a mVenus tagged with a membrane localization sequence

   Depositor

   Dorus Gadella

   Article

   van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

   Insert

   Lck (MGCVCSSNPELPVAT)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRU319

   Plasmid

   #167686

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-HkCas12a-2AeGFP

   Plasmid

   #123634

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   HkCas12a
   

   Use

   Tags

   3xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDGE1109

   Plasmid

   #202773

   Purpose

   CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

   Inserts

   FAST marker
   pRPS5a:TREX2-zCas9_ttriple
   ccdB cassette (BsaI-excisable)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOCC114

   Plasmid

   #118904

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with TEV protease, two glycines

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRU292

    Plasmid

    #167687

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTC223

    Plasmid

    #70019

    Purpose

    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (Cas9/sgRNA) + Donor + GVR
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRU61

    Plasmid

    #167692

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::asCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NmeCas9_3XNLS_pMCSG7

    Plasmid

    #120078

    Purpose

    Bacterial expression of wtNmeCas9 with 3X NLS

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    NmeCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOPTN425-577-EGFP

    Plasmid

    #68843

    Purpose

    deleted OPTN was inserted into pEGFP-N1 backbone

    Depositor

    Beatrice Yue

    Article

    Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 425-577 only

    Promoter

    CMV

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pOPTN1-424-EGFP

    Plasmid

    #68840

    Purpose

    deleted OPTN was inserted into pEGFP-N1 backbone

    Depositor

    Beatrice Yue

    Article

    Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-424 only

    Promoter

    CMV

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113342

    Purpose

    Expresses dCas9-p300 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    dCas9-p300core-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-neo-Strep_Flag_Cterm

    Plasmid

    #102645

    Purpose

    Epitope tag c-terminus with SBP-Flag moiety

    Depositor

    Michael E. Wright

    Article

    Hsiao et al BMC Cancer. 2015 Mar 31;15:204. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pE478G-EGFP

    Plasmid

    #68848

    Purpose

    mutated OPTN was inserted into pEGFP-N1 backbone

    Depositor

    Beatrice Yue

    Article

    Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E478G, associated with ALS

    Promoter

    CMV

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113344

    Purpose

    Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    VP64-dCas9-p300core-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC1750

    Plasmid

    #127984

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge