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Showing: 2551 - 2600 of 30005 results

  7. pPuroR-MCS-LexA-VP16-MiniWhite

    Plasmid
    #165898
    Purpose
    Puromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable Empty Enhancer Driver
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165898-plasmid-map-sequence-id-331224

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  9. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

    Plasmid
    #172296
    Purpose
    In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing
    Depositor
    Patrik Verstreken
    Insert
    Cas9n-RT-T2A-PuroR
    Use
    CRISPR
    Tags
    Expression
    Mutation
    H840A (Cas9)
    Promoter
    T7 promoter A-initiating
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172296-plasmid-map-sequence-id-340322

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  10. 970 pBabe puroL PTEN R130G

    Plasmid
    #10783
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    R130G
    Promoter
    Available since
    Sept. 27, 2005
    Availability
    Academic Institutions and Nonprofits only

  15. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid
    #207760
    Purpose
    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207760-plasmid-map-sequence-id-413789

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  18. integrin beta6 Y774F pBabe puro

    Plasmid
    #13599
    Depositor
    Dean Sheppard
    Insert
    integrin beta 6 Y774F (ITGB6 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Y774F
    Promoter
    Available since
    Jan. 8, 2007
    Availability
    Academic Institutions and Nonprofits only

  24. Ef1a-somBiPOLES-mCerulean-IRES-Puro

    Plasmid
    #192585
    Purpose
    lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    Lentiviral
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-alpha
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192585-plasmid-map-sequence-id-379281

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  27. pLKO-Tet-puro-hARAF-shRNA-1

    Plasmid
    #185370
    Purpose
    For tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAF
    Depositor
    Ayaz Najafov
    Insert
    ARAF (ARAF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185370-plasmid-map-sequence-id-363837

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  28. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

    Plasmid
    #171801
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-hgp100-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171801-plasmid-map-sequence-id-337972

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  30. integrin alpha9 EGFP pBabe puro

    Plasmid
    #13605
    Depositor
    Dean Sheppard
    Insert
    integrin alpha 9 (ITGA9 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 8, 2007
    Availability
    Academic Institutions and Nonprofits only

  40. pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc

    Plasmid
    #161587
    Purpose
    expression of Myc-tagged mouse Lrrk2 without GTPase domain
    Depositor
    Maik Hintze
    Insert
    Lrrk2 (Lrrk2 Mouse)
    Use
    Tags
    Myc tag and StrepII
    Expression
    Mammalian
    Mutation
    deletion: AA1023-1879
    Promoter
    CMV
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    161587-plasmid-map-sequence-id-318592

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  41. pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc

    Plasmid
    #161586
    Purpose
    expression of Myc-tagged mouse Lrrk2 without kinase domain
    Depositor
    Maik Hintze
    Insert
    Lrrk2 (Lrrk2 Mouse)
    Use
    Tags
    Myc tag and StrepII
    Expression
    Mammalian
    Mutation
    deletion: AA1871-2540
    Promoter
    CMV
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    161586-plasmid-map-sequence-id-318591

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  43. pIVTR(T7A)-CRE-T2A-PuroR

    Plasmid
    #172298
    Purpose
    In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes
    Depositor
    Patrik Verstreken
    Insert
    CRE-T2A-PuroR
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter A-initiating
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172298-plasmid-map-sequence-id-340323

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  44. Direct-seq_lentiGuide-Puro-Tail-8A8G

    Plasmid
    #157981
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (Tail-8A8G)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    157981-plasmid-map-sequence-id-309444

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Showing: 2551 - 2600 of 30005 results