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Plasmid#195507Purposepiggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sitesDepositorInsertPuromycin
UseCRISPR; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-miRFP703-eDHFR(69K6)-cRaf
Plasmid#184717PurposeiRafDepositorInsertmiRFP703-eDHFR(69K6)-cRaf (RAF1 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-4493NES(Booster-PKA)
Plasmid#184711PurposePKA biosensorDepositorInsertBooster-PKA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-Puro_TAL1-short
Plasmid#210641PurposeOverexpression of TAL1-shortDepositorInsertTAL1-short (TAL1 Human)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNKX2-5-T2A-GFP-PGK-Puro
Plasmid#203607PurposeDonor plasmid to create 3' knockin of NKX2-5 with T2A-GFPDepositorInsertNKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)
UseHuman targetingTagsExpressionMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-V1AUC-DelStem-CD20-Puro
Plasmid#209758PurposeLentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1; variant 1 mRNA isoform with mutations to disrupt the uORFs and the stem-loop within the 5'-UTR.DepositorInsertMS4A1 (MS4A1 Human)
UseLentiviralTagsExpressionMutationMutations to disrupt the uORFs and the stem-loop …PromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S351A
Plasmid#206913PurposeExpression of mOct4 S351A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS351APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 T87A
Plasmid#206921PurposeExpression of mOct4 T87A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationT87APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S100A
Plasmid#206920PurposeExpression of mOct4 S100A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS100APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S102A
Plasmid#206919PurposeExpression of mOct4 S102A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS102APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 T228A
Plasmid#206918PurposeExpression of mOct4 T228A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationT228APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S229A
Plasmid#206917PurposeExpression of mOct4 S229A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS229APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S281A
Plasmid#206916PurposeExpression of mOct4 S281A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS281APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S282A
Plasmid#206915PurposeExpression of mOct4 S282A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS282APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLAG-puro-mOct4 S347A
Plasmid#206914PurposeExpression of mOct4 S347A mutationDepositorInsertOct4 (Pou5f1 Mouse)
UseTagsFLAGExpressionMammalianMutationS347APromoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro
Plasmid#201677PurposeEBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassetteDepositorInsertMIR302-3g-EEA-2g-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-MIR302-7guides-PGK-Puro
Plasmid#201960PurposeEBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassetteDepositorInsertMIR302-7g-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mBltp1 (KIAA1109) - 1
Plasmid#198489Purposelentiviral stable expression of mBltp1 (KIAA1109) gRNA 1DepositorInsertmBltp1 (4932438A13Rik Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mBltp1 (KIAA1109) - 2
Plasmid#198490Purposelentiviral stable expression of mBltp1 (KIAA1109) gRNA 2DepositorInsertmBltp1 (4932438A13Rik Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSK-mU6-Cj-sgRNA-SV40-puro
Plasmid#192128PurposeExpresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance geneDepositorInsertCj-SgRNA scaffold
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgCtrl_EF1a-Puro-T2A-BFP
Plasmid#195500PurposeLentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequenceDepositorInsertsgRNA
UseCRISPR and LentiviralTagsBFP, Puro, and T2AExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV puro PRDM14 ΔSET domain
Plasmid#193680PurposeConstitutive retroviral expression of PRDM14DepositorInsertPRDM14 (PRDM14 Human)
UseRetroviralTagsExpressionMutationDeletion of SET domainPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hMYOD1 gRNA_1-MS2-Puro
Plasmid#192673PurposeLentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcriptionDepositorInsertHuman MYOD1 activating gRNA #1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hASCL1 gRNA_1-MS2-Puro
Plasmid#192670PurposeLentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcriptionDepositorInsertHuman ASCL1 activating gRNA #1 (ASCL1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hIL1RN gRNA_1-MS2-Puro
Plasmid#192677PurposeLentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcriptionDepositorInsertHuman IL1RN activating gRNA #1 (IL1RN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjOSR1-EGFP-PGK-Puro
Plasmid#186170PurposeKnock-in gene targeting in marmoset cells at OSR1 locusDepositorInsertEGFP
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PL752-hGH-polyA-PGK-puroR
Plasmid#191682PurposeDonor plasmid backbone PGK-puroRDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pan-Neurofascin (extracellular) scFv [A12/18]
Plasmid#190485PurposeMammalian Expression of Pan-Neurofascin (extracellular) scFV. Derived from hybridoma A12/18.DepositorInsertPan-Neurofascin (extracellular) (Rattus norvegicus) recombinant scFV (Nfasc Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjFOXL2-EGFP-PGK-Puro
Plasmid#186167PurposeKnock-in gene targeting in marmoset cells at FOXL2 locusDepositorInsertEGFP
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-HA-FLAG-AU1
Plasmid#162118PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to HA-FLAG-AU1
UseLentiviralTagsHA-FLAG-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-VSVg-ProtC-HA
Plasmid#162103PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to VSVg-ProtC-HA
UseLentiviralTagsVSVg-ProtC-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-VSVg-HA-FLAG
Plasmid#162106PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to VSVg-HA-FLAG
UseLentiviralTagsVSVg-HA-FLAGExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-VSVg-HA-AU1
Plasmid#162107PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to VSVg-HA-AU1
UseLentiviralTagsVSVg-HA-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-HA-FLAG
Plasmid#162112PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to StrepTagII-HA-FLAG
UseLentiviralTagsStrepTagII-HA-FLAGExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-HA-AU1
Plasmid#162113PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to StrepTagII-HA-AU1
UseLentiviralTagsStrepTagII-HA-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-ProtC-HA-FLAG
Plasmid#162115PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to ProtC-HA-FLAG
UseLentiviralTagsProtC-HA-FLAGExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-ProtC-HA-AU1
Plasmid#162116PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to ProtC-HA-AU1
UseLentiviralTagsProtC-HA-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-VSVg-HA-FLAG
Plasmid#162086PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to VSVg-HA-FLAG
UseLentiviralTagsVSVg-HA-FLAGExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-VSVg-HA-AU1
Plasmid#162087PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to VSVg-HA-AU1
UseLentiviralTagsVSVg-HA-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-HA
Plasmid#162089PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to StrepTagII-ProtC-HA
UseLentiviralTagsStrepTagII-ProtC-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-StrepTagII-HA-FLAG
Plasmid#162092PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to StrepTagII-HA-FLAG
UseLentiviralTagsStrepTagII-HA-FLAGExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-StrepTagII-HA-AU1
Plasmid#162093PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to StrepTagII-HA-AU1
UseLentiviralTagsStrepTagII-HA-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-ProtC-HA-FLAG
Plasmid#162095PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to ProtC-HA-FLAG
UseLentiviralTagsProtC-HA-FLAGExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-ProtC-HA-AU1
Plasmid#162096PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to ProtC-HA-AU1
UseLentiviralTagsProtC-HA-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-HA-FLAG-AU1
Plasmid#162098PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to HA-FLAG-AU1
UseLentiviralTagsHA-FLAG-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-HA
Plasmid#162080PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to VSVg-StrepTagII-HA
UseLentiviralTagsVSVg-StrepTagII-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-HA
Plasmid#162083PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to VSVg-ProtC-HA
UseLentiviralTagsVSVg-ProtC-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2ABFP-W
Plasmid#163175PurposeLentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tagDepositorInsertWWTR1 (WWTR1 Human)
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2ABFP-W
Plasmid#163174PurposeLentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tagDepositorInsertWWTR1 (WWTR1 Human)
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMERVK9c_1 (inducible)
Plasmid#189957PurposeshRNA mediated knockdownDepositorInsertERV_MMERVK9c
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only