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Plasmid#221470PurposeGateway cloning of mCherry overexpressionDepositorInsertmCherry
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA179
Plasmid#215939PurposeFragmid fragment: (guide cassette) short sequence with no known functionDepositorHas ServiceCloning Grade DNAInsertFiller
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
12URA-B-ScTop2∆CTD
Plasmid#214924PurposeYeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)DepositorInserttopoisomerase II
UseTags6x His-TEVExpressionYeastMutationDeleted amino acids 1178-1428PromoterGal 1/10Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP64
Plasmid#212897PurposeExpresses the Priestia megaterium LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationP450BM3 MERO1 (V78A, A82G, F87V, P142S, T175I, A1…PromoterPcymRCAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBR BsmAIM
Plasmid#206206PurposeConstitutive expression of BsmAIM methyltransferaseDepositorInsertBsamAIM
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_R166QTS
Plasmid#191298PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F/G121V DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-HA-BioID2-SAPAP3
Plasmid#176857PurposeExpresses SAPAP3 protein fused with BioID2 at the N-terminus in neuronsDepositorInsertHA-BioID2-SAPAP3
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-ADCK5-V5-miniTurbo_IDG-K
Plasmid#189909PurposeGateway destination clone of ADCK5 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertADCK5 (ADCK5 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-COQ8A-V5-miniTurbo_IDG-K
Plasmid#189885PurposeGateway destination clone of COQ8A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertCOQ8A (COQ8A Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mPpp3cc-FLAG-6XHIS
Plasmid#179160PurposeExpression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
UseTags6XHIS and FLAGExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab32b
Plasmid#140885PurposeExpression of zebrafish GFP-tagged Rab32b in mammalian cells.DepositorInsertRab32b (rab32b Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP64-GFP-ePDZ-CAAX
Plasmid#135603PurposeExpression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratusDepositorInsertLifeact-GFP-ePDZ-CAAX
UseIn vitro transcription (mrna synthesis)TagsCAAX, EGFP, and ePDZExpressionMutationPromoterUnknownAvailable sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-membrane-mCherry-LOV
Plasmid#135599PurposeExpression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratusDepositorInsertmembrane-mCherry-LOV
UseIn vitro transcription (mrna synthesis)TagsLOV and mCherryExpressionMutationPromoterUnknownAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-19a-3p
Plasmid#103323PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-19a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-19a-3p target (MIR19A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
DGKQ gRNA (BRDN0001162275)
Plasmid#77377Purpose3rd generation lentiviral gRNA plasmid targeting human DGKQDepositorInsertDGKQ (DGKQ Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP3K3 gRNA (BRDN0001148097)
Plasmid#76016Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K3DepositorInsertMAP3K3 (MAP3K3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_EGFP_Donor
Plasmid#178088PurposeHDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mGold2t-Tubulin-C-18
Plasmid#231779PurposeVisualization of tubulin in mammalian cells using mGold2tDepositorInsertmGold2t-Tubulin-C-18 (TUBA1B Human)
UseTagsmGold2sExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
MP6
Plasmid#69669PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi CDA1
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-mCherry
Plasmid#137132PurposeIntersectional viral expression of mCherry in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-HA
Plasmid#128034PurposeMammalian expression vector with N terminal HA tagDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3Luc-5XNF-kappaB
Plasmid#185695PurposeReporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').DepositorInsertNF-kappaB (NFKB1 Human)
UseLuciferaseTagsExpressionMutationPromoterNF-kappaB binding siteAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pBOB-EF1-FastFUCCI-Puro
Plasmid#86849Purpose3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selectionDepositorInsertsmKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1 and PGKAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
sortase A pentamutant (eSrtA) in pET29
Plasmid#75144Purposeevolved sortase A (eSrtA) pentamutant with improved kinetics and activityDepositorInsertsortase A pentamutant
UseTags6x HisExpressionBacterialMutationP94R, D160N, D165A, K190E, K196T. Deleted amino a…PromoterT7Available sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cas3
Plasmid#209427PurposePlasmid for CASCADE-Cas3 based genome engineering of streptomycetesDepositorInsertcodon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; crRNA under control of PermE*Available sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dR8.2 dvpr
Plasmid#8455Purpose2nd* generation lentiviral packaging plasmid. (*See comments section.) Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
mCherry
Plasmid#176016PurposeMammalian expression of mCherry. Parton lab clone DFQDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi 41BBL-CD86 mIL-15
Plasmid#183245PurposeExpression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cellsDepositorInsert41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only