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pLX313-TP53-WT
Plasmid#118014PurposeConstitutive expression of wild-type human Tumor Protein p53 (TP53)DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLBW cox8 EGFP mCherry
Plasmid#78520PurposeFluorescent mitophagy reporterDepositorInsertnone
UseRetroviralTagscox8 EGFP mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGH335_MS2-AID*Δ-Hygro
Plasmid#85406Purposelentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable markerDepositorInsertMS2-AID*Δ and Hygromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV.CAP-B10
Plasmid#175004Purposenon-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation systemDepositorInsertSynthetic construct isolate AAV.CAP-B10 VP1 gene
UseAAVTagsExpressionMammalianMutation7 amino acid substitution between VP1 452 and VP1…Promoterp41Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRAv3:minP-GFP
Plasmid#109036PurposeMPRA v3 minimal promoter with GFP donorDepositorInsertemGFP
UseTagsExpressionMammalianMutationPromoterminPAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon DREADD Gi-mCherry
Plasmid#177672PurposeExpresses DREADD Gi in Cre and Flp expressing cellsDepositorHas ServiceAAV8InsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianMutationPromoternEFAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 neo
Plasmid#13425Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.DepositorInsertnon-hairpin 18bp
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceDec. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-VN173
Plasmid#22010PurposeMammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assaysDepositorInsertVN173
UseTagsFLAGExpressionMammalianMutationVenus residus 1-172PromoterAvailable sinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6-150
Plasmid#86918PurposeFluorescent reporter for ER calcium signalingDepositorInsertER-GCaMP6-150
UseLentiviralTagsExpressionMammalianMutationK78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…PromoterCAMKIIAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRE4-Azi
Plasmid#105829PurposePlasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cellsDepositorInsertsHumanized EAziRS
4 copies of the U6-BstYam tRNA cassette
UseTagsExpressionMammalianMutation(Y37L, D182S, F183M, L186A, D265R)TyrRSPromoterPGK and U6Available sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGs-RFP
Plasmid#127347PurposeExpresses RFP constitutively via the CAGGS promoter in mammalian cellsDepositorInsertRed Fluorescent Protein
UseTagsExpressionMammalianMutationPromoterCAGGS PromoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TALEN-L
Plasmid#59025PurposeTALEN designed to target the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-TALEN-L
UseAAV and TALENTags3xFlag and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TALEN-R
Plasmid#59026PurposeTALEN designed to target the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-TALEN-R
UseAAV and TALENTags3xFlag and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFP
Plasmid#32395DepositorInsertGFP
UseAAV; Adeno-associated virusTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1-Cre-PGK-Puro
Plasmid#108543PurposeLentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoterDepositorInsertscre recombinase
Puromycin
UseTagsExpressionMutationPromoterEF1apha and PGKAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-pSu9-HaloTag7-mGFP
Plasmid#184905PurposeStable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activityDepositorInsertPresequence of Fo-ATPase subunit 9
UseTagsHaloTag7 and monomeric EGFPExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7II-2N4Rtau
Plasmid#177653PurposeRecombinant protein synthesis of human TauDepositorInsertTau (2N4R) (MAPT Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
tetO.Nfib.Hygro
Plasmid#117271PurposeExpresses Nfib and hygromycin resistance gene under control of tetO sequence.DepositorInsertNfib (Nfib Mouse)
UseLentiviralTagsIRES and hygromycin resistant cassette (C termina…ExpressionMammalianMutationPromotertet-onAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAdTrack-PPARdelta
Plasmid#16529DepositorInsertPPAR delta (PPARD Human)
UseAdenoviralTags2x HA (YDVPDYAS)ExpressionMammalianMutationPromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only
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