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Plasmid#74005PurposegRNA for B108DepositorInsertgRNA
UseCRISPRTagsnoneExpressionMutationPromoterU6Available sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
B53
Plasmid#74006PurposegRNA for B108DepositorInsertgRNA
UseCRISPRTagsnoneExpressionMutationPromoterU6Available sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
B31
Plasmid#74007PurposegRNA for B108DepositorInsertgRNA
UseCRISPRTagsnoneExpressionMutationPromoterU6Available sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-Mb-crRNA
Plasmid#78959PurposeCloning vector for expression of MbCpf1 crRNA. It contains BsmB1 site for cloning.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide_puro-P2A-mNeonGreen
Plasmid#221464PurposeLentiviral expression of an sgRNA with mNeonGreen fluorescent markerDepositorInsertmNeonGreen
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6, EF-1aAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hCas9D10A
Plasmid#52101PurposeThe expression vector for humanized nickase Cas9 (Cas9D10A) under CAG promoterDepositorInserthCas9D10A
UseTagsSV40 NLSExpressionMammalianMutationD10APromoterCAGAvailable sinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKSB- 3xP3-DsRedSV40 GGGG AAGA
Plasmid#174532Purpose3xP3-DsRed fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-DsRed BsaI cassette
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pY120 (pcDNA3.1-huFbCpf1)
Plasmid#92296PurposeExpression of humanized FbCpf1DepositorInserthuFbCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY126 (pcDNA3.1-huBoCpf1)
Plasmid#92302PurposeExpression of humanized BoCpf1DepositorInserthuBoCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY123 (pcDNA3.1-huCMaCpf1)
Plasmid#92299PurposeExpression of humanized CMaCpf1DepositorInserthuCMaCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY114 (pcDNA3.1-huPgCpf1)
Plasmid#92273PurposeExpression of humanized PgCpf1DepositorInserthuPgCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY122 (pcDNA3.1-huCPbCpf1)
Plasmid#92298PurposeExpression of humanized CPbCpf1DepositorInserthuCPbCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 MS2 (GB2665)
Plasmid#160597PurposeCDS of MS2 protein with B3 nomenclatureDepositorInsertMS2
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE
Plasmid#65821PurposeControl guide RNA plasmid E. coli , low phosphate inductionDepositorInsertcontrol guideRNA
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide-BlastR
Plasmid#196970PurposeLentiviral acceptor vector for pegRNAs with blasticidin resistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterhU6Available sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
HCP9
Plasmid#166111PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2DepositorInsertHta2-sg18 (HTA2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS15_MATz
Plasmid#166100PurposeExpression of dual gRNA targeting the MAT locusDepositorInsertMAT Z1
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7 RNA POL
Plasmid#138352PurposeExpressing T7 RNA Polymerase in mammalian cellsDepositorInsertT7 RNA POL
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP-seq-opti-dsRed
Plasmid#201999PurposeBased on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsdsRedExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR6K-GFP-RFP
Plasmid#199652PurposeR6K backbone with Golden Gate (GG) cloning sitesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterJ23119 promoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only