Addgene

Showing: 10301 - 10320 of 18171 results

Results per page:

Sort results by:

pmiRGLO-KIAA2013-201

Plasmid

#117348

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR KIAA2013-201

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRGLO-FAM102A-203

Plasmid

#117344

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR FAM102A-203

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-HA/Bmal L95E/L150E/W427A

Plasmid

#47352

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Ha

Expression

Mammalian

Mutation

L95E/L150E/W427A

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pmiRGLO-GRM4-202

Plasmid

#117347

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR GRM4-202

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MLS-mir-17-92-Mut20

Plasmid

#64095

Purpose

expresses a mutated version of mir-17-92 (mutation of miR-20 seed)

Depositor

Lin He

Article

Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

Insert

miR-17-92 (MIR17HG Human)

Use

Tags

Expression

Mammalian

Mutation

mutation of miR-20 seed sequence

Promoter

MSCV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
TRCN0000072213

Plasmid

#78159

Purpose

shRFP control

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

RFP

Use

RNAi

Tags

Expression

Mutation

Promoter

hU6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-4T3-ZO-2-1st-PDZ-delta-NLS

Plasmid

#27425

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Deletion of NLS-Nuclear Localization Signal: Nucl…

Promoter

Available since

May 18, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-chCARex

Plasmid

#87233

Purpose

bacterial expression of GST-CAR extracellular domain

Depositor

Fritz Rathjen

Article

Patzke et al J Neurosci. 2010 Feb 24;30(8):2897-910.

Insert

CXADR (CXADR Chicken)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pVL45 - pPXY>>

Plasmid

#116011

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pPXY:GR-LhG4:tPXY:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Use

Tags

HDEL and SP(ER)

Expression

Plant

Mutation

Promoter

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_CD2AP-1/3

Plasmid

#91398

Purpose

Protein expression and purification of human SH3 domain construct CD2AP-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CD2AP-1/3 (CD2AP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_EPS8-1/1

Plasmid

#91254

Purpose

Protein expression and purification of human SH3 domain construct EPS8-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

EPS8-1/1 (EPS8 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-HA/Bmal W427A

Plasmid

#47349

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Ha

Expression

Mammalian

Mutation

W427A

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pGEX SH2D4B-SH2

Plasmid

#46482

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2D4B (Sh2d4b Mouse)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 311-431

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX SH2D5-SH2

Plasmid

#46483

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2D5 (Sh2d5 Mouse)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 290-399

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_BAIAP2L2-1/1

Plasmid

#91393

Purpose

Protein expression and purification of human SH3 domain construct BAIAP2L2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BAIAP2L2-1/1 (BAIAP2L2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX SH2-B-SH2 RA

Plasmid

#46480

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2-B RK (SH2B1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 515-639; R555A mutation in t…

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLAG-WTS-1

Plasmid

#66856

Purpose

Express FLAG-tagged C.elegans WTS-1 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

WTS-1 (wts-1 Nematode)

Use

Tags

FLAG

Expression

Mammalian

Mutation

E153G and D871G mutations in WTS1 compared to Gen…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pIDS-RESTmut full-length

Plasmid

#109162

Purpose

Encodes human full-length REST/NRSF C402S to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length REST/NRSF (REST Human)

Use

Tags

Expression

Insect

Mutation

Cys402Ser

Promoter

p10

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmMextli

Plasmid

#79262

Purpose

Expresses EGFP-tagged DmMextli in S2 cells

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli (mxt Fly, NM_164598)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5 FRT TO myc Separase delta 55/S1126A

Plasmid

#59825

Purpose

Allows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

delta 55/S1126A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge