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RV-mCD4-HH5-1

Plasmid

#124961

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-1 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-1

Use

retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pD864-LacZ

Plasmid

#109380

Purpose

Rhamnose inducible LacZ expression. Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pRHAM-LacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2AGAL4

Plasmid

#125213

Purpose

Artificial phase 2 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL4

Use

Other

Tags

T2A

Expression

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLPCX roGFP2-Prx2

Plasmid

#169230

Purpose

Genetically encoded fluorescent H2O2 sensor roGFP2-Prx2 for cytosolic expression in mammalian cells (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

roGFP2-Prx2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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Gal:mCherry-CRY2-uTEV1Δ(220-242)

Plasmid

#135452

Purpose

Yeast expression of uTEV1Δ fused to CRY2 and mCherry

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

mCherry-CRY2-TEV1Δ(220-242)

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

Gal

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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mFGF8a

Plasmid

#22088

Depositor

Gail Martin

Article

Crossley et al Development. 1995 Feb . 121(2):439-51.

Insert

FGF8 (Fgf8 Mouse)

Use

Bacterial

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pBS_KS_attB2_SA(0)_T2AGAL4

Plasmid

#125211

Purpose

Artificial phase 0 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL4

Use

Other

Tags

T2A

Expression

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn1beta AS4(-)

Plasmid

#58267

Purpose

Expression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn1beta AS4(-) (Nrxn1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

see comments

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSBtet-Bla/PAG1-TurboID-GAI

Plasmid

#198217

Purpose

Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling

Depositor

Eric Davis

Insert

PAG1 (PAG1 Human)

Use

Transposon

Tags

3’ part of TurboID, starting with G257 and amino …

Expression

Mammalian

Mutation

None

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(1)_T2AGAL4

Plasmid

#125212

Purpose

Artificial phase 1 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL4

Use

Other

Tags

T2A

Expression

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pX459_gRNA-ROSA26_hspCas9

Plasmid

#193310

Purpose

Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

not applicable

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pOGG011

Plasmid

#113985

Purpose

Vector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

pBBR1

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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SPDK3184

Plasmid

#97405

Purpose

binary construct of cytosolic sfCFP1-10 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfCFP1-10

Use

Tags

cytosol

Expression

Plant

Mutation

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCIneo-hCHD7-Kozak ATG 3' HA-bGH polyA

Plasmid

#89460

Purpose

constitutive expression of human CHD7 in mammalian cells

Depositor

Peter Scacheri

Insert

CHD7 (CHD7 Human)

Use

Tags

hinge + HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMM117

Plasmid

#127212

Purpose

BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, sgRNA

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTRANS_101

Plasmid

#91199

Purpose

Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn2beta AS4(-)

Plasmid

#58268

Purpose

Expression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn2beta AS4(-) (Nrxn2 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pYFAC-pyroA

Plasmid

#168976

Purpose

Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; pyroA

Depositor

Yit Heng Chooi

Article

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Expression in aspergillus nidu…

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pTRANS_233

Plasmid

#91122

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-LSSA12

Plasmid

#186545

Purpose

LSSA12 fluorescent protein (ex/em = 398/511 nm): cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSA12

Use

Tags

Expression

Mammalian

Mutation

hfYFP-G65S/Y203I/Q204E/E222D/R223F

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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