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Plasmid#124961PurposeExpress the Helicobacter hepaticus specific TCRa/b HH5-1 in T cell hybridoma cellsDepositorInsertHH5-1
UseretroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pD864-LacZ
Plasmid#109380PurposeRhamnose inducible LacZ expression. Used to test burden response in E. coliDepositorInsertpRHAM-LacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(2)_T2AGAL4
Plasmid#125213PurposeArtificial phase 2 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGAL4
UseOtherTagsT2AExpressionMutationPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLPCX roGFP2-Prx2
Plasmid#169230PurposeGenetically encoded fluorescent H2O2 sensor roGFP2-Prx2 for cytosolic expression in mammalian cells (for stable cell line generation)DepositorInsertroGFP2-Prx2
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gal:mCherry-CRY2-uTEV1Δ(220-242)
Plasmid#135452PurposeYeast expression of uTEV1Δ fused to CRY2 and mCherryDepositorInsertmCherry-CRY2-TEV1Δ(220-242)
UseTagsmCherryExpressionYeastMutationPromoterGalAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pBS_KS_attB2_SA(0)_T2AGAL4
Plasmid#125211PurposeArtificial phase 0 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGAL4
UseOtherTagsT2AExpressionMutationPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-rat Nrxn1beta AS4(-)
Plasmid#58267PurposeExpression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)DepositorInsertNrxn1beta AS4(-) (Nrxn1 Rat)
UseTagsHAExpressionMammalianMutationsee commentsPromoterCAGAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla/PAG1-TurboID-GAI
Plasmid#198217PurposeTransposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labelingDepositorInsertPAG1 (PAG1 Human)
UseTransposonTags3’ part of TurboID, starting with G257 and amino …ExpressionMammalianMutationNonePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(1)_T2AGAL4
Plasmid#125212PurposeArtificial phase 1 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGAL4
UseOtherTagsT2AExpressionMutationPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX459_gRNA-ROSA26_hspCas9
Plasmid#193310PurposeCas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)DepositorInsertCas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
UseCRISPRTagsExpressionMammalianMutationnot applicablePromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOGG011
Plasmid#113985PurposeVector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertpBBR1
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
SPDK3184
Plasmid#97405Purposebinary construct of cytosolic sfCFP1-10 tag for plant expressionDepositorInsertsfCFP1-10
UseTagscytosolExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-hCHD7-Kozak ATG 3' HA-bGH polyA
Plasmid#89460Purposeconstitutive expression of human CHD7 in mammalian cellsDepositorInsertCHD7 (CHD7 Human)
UseTagshinge + HAExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM117
Plasmid#127212PurposeBeYDV replicon with constitutive Luc expression, sgRNA targeting PDSDepositorInsertLuc, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_101
Plasmid#91199PurposeTransformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: BeYDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-rat Nrxn2beta AS4(-)
Plasmid#58268PurposeExpression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)DepositorInsertNrxn2beta AS4(-) (Nrxn2 Rat)
UseTagsHAExpressionMammalianMutationPromoterCAGAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-pyroA
Plasmid#168976PurposeFilamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; pyroADepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus nidu…TagsExpressionMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_233
Plasmid#91122PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-LSSA12
Plasmid#186545PurposeLSSA12 fluorescent protein (ex/em = 398/511 nm): cytosolic expression in mammalian cellsDepositorInsertLSSA12
UseTagsExpressionMammalianMutationhfYFP-G65S/Y203I/Q204E/E222D/R223FPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only