-
Plasmid#112061PurposeGenetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.DepositorInsertNES-ZapCV5
UseTagsNuclear Export Signal (NES) derived from HIV-1ExpressionMammalianMutationECFP gene is truncated 36 bp at 3' end. Venu…PromoterCBAAvailable sinceJuly 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-rIgG-Fc2-ICAM1
Plasmid#156462PurposeExpresses a rabbit Fc-ICAM1 fusion protein in mammalian cellsDepositorInsertIntercellular Adhesion Molecule 1 (Icam1 Mouse)
UseTagsICAM1-rFcExpressionBacterial and MammalianMutationendogenous signal peptide replaced with IL2 signa…PromoterhEF1-HTLCAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP GFP-MOSPD2 WT
Plasmid#186467PurposeExpression of human MOSPD2 fused to EGFP in mammalian cellsDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB[U6-3 wex2 PUb-DsRed]
Plasmid#169010PurposeFor germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.DepositorInsertwhite ex2 sgRNA
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster U6:3Available sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV_mIFP IRES EGFP
Plasmid#72443PurposeExpressing mIFP and EGFP in mammalian cellsDepositorInsertmIFP T2A HO1 IRES EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-NES-ZapCV2 (cpV143)
Plasmid#112060PurposeGenetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)DepositorInsertNES-ZapCV2
UseTagsNuclear Export Signal (NES) derived from HIV-1ExpressionMammalianMutationECFP gene is truncated 36 bp at 3' end. Venu…PromoterCMVAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-kappa-HA-dL5-BKa
Plasmid#73212PurposeExpresses HA-dL5(E52D)-BKa (KCNMA1, mSlo alpha subunit) in mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)DepositorInsertBKalpha (Kcnma1 Mouse)
UseTagsdL5**ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-KozATG-dH6.2-2XG4S-actin
Plasmid#73263PurposeExpresses dH6.2-actin fusion within mammalian cells. (dH6.2, FAP)DepositorInsertKozATG-dH6.2-2XG4S-actin (ACTB Human, Synthetic)
UseTagsThe FAP and actin are fused with 2 copies of a G4…ExpressionMammalianMutationThe dH6.2 FAP has one cysteine in each domain cha…PromoterCMVAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Drd1a->M71-IRES-tauGFP ACNF TV
Plasmid#105073PurposeTargeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-447 of Drd1a and an IRES-tauGFP followed by ACNF cassetteDepositorUseMouse TargetingTagsExpressionMutationDrd1a coding sequence followed by IRES-tauGFP ACNFPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pMXS-IRES-BLAST flag-CAD R2187A
Plasmid#169827PurposeExpresses C-terminal flag-tagged CAD with mutation at reported trimerization interface of the ATCase domain of CADDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationR2187A, T456A S1406A S1859A; TCCC -> AGTC sile…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD H1734A H1741A
Plasmid#169830PurposeExpresses C-terminal flag-tagged CAD with mutations at reported dimerization interface of the DHOase domainDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationH1734A H1741A; TCCC -> AGTC silent mutations a…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD delta Loop1
Plasmid#169831PurposeExpresses C-terminal flag-tagged CAD with deletion of amino acids F1366 - P1376 in allosteric domainDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationDeletion of amino acids F1366 - P1376; TCCC ->…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD delta Loop2
Plasmid#169832PurposeExpresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 in allosteric domainDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationDeletion of amino acids R1398 - S1407; TCCC ->…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
-
EF1a-Cas9-2XNES-2ERT2
Plasmid#120551PurposeExpress Cas9, 2xNES and 2 tandem ERT2 in mammalian cellsDepositorInsertCas9, NES and ERT2
UseLentiviralTagsExpressionMammalianMutationPromoterEF1αAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-HSP70
Plasmid#163790PurposeExpresses mScarlet-Hsp70 in mammalian cellsDepositorInsertHSP70 (HSPA1A Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_AOX-FLAG
Plasmid#177984PurposeAll-in-one dox-inducible expression of Alternative OxidaseDepositorInsertAOX
UseLentiviralTagsFLAGExpressionMammalianMutationCodon optimized cDNA for expression in human cellsPromoterTREAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MtAcKRS (human-opti)
Plasmid#164195PurposeTo express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansionDepositorInsertMtAcKRS-478(human-opti)
UseSynthetic BiologyTagsExpressionMammalianMutationD76G; L325M; L329I; Y330F; L333A; C372FPromoterCMVAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only