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Plasmid#115463PurposeConstitutive lentiviral expressionDepositorInsertshMBNL3-0455 (MBNL3 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1alpha-IRES-EGFP-3XFlag-hCEP164
Plasmid#122966PurposeExpresses 3XFlag-tagged human CEP164 in mammalian cellsDepositorInsertCEP164 (CEP164 Human)
UseLentiviralTags3XFlagExpressionMutationPromoterEF1 alphaAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP650
Plasmid#135000PurposeVP64-m6A Reader. Activates transcription near Gm6ATC sites. pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoRDepositorInsertpUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR
UseLentiviral and Synthetic BiologyTagsDpnI(aa146-254), V5 tag, and VP-64ExpressionMammalianMutationDpnI truncated to aa146-254PromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-182-Decoy.hPGK.GFP.WPRE
Plasmid#46596DepositorInsertmiR-182 Decoy
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti GW V5 Eco/Dam mse EMD
Plasmid#182675PurposeLentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.DepositorInsertEmerin (Emd Mouse)
UseLentiviralTagsE. Coli Dam and V5ExpressionMammalianMutationPromoterHSPAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgGPT2_9
Plasmid#163456Purposelentiviral vector expressing Cas9 and an sgRNA targeting GPT2DepositorInsertsgRNA 9 targeting GPT2 (GPT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
ARAF gRNA (BRDN0001147899)
Plasmid#75660Purpose3rd generation lentiviral gRNA plasmid targeting human ARAFDepositorInsertARAF (ARAF Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ARAF gRNA (BRDN0001148605)
Plasmid#75662Purpose3rd generation lentiviral gRNA plasmid targeting human ARAFDepositorInsertARAF (ARAF Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti-Cas9-VQR-GFP_activity_reporter
Plasmid#87156PurposeThis lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).DepositorInsertGFP and sgRNA targeting GFP (NGA restricted)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-UMPS (Y37A, R155A)-3xFLAG
Plasmid#87976PurposeExpression of UMPS (Y37A, R155A) in mammalian cellsDepositorInsertUMPS (Y37A, R155A) (UMPS Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationY37A, R155APromoterCMVAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO-PHB-6
Plasmid#122232PurposeExpresses shRNA targeting the coding sequence of human PHBDepositorInsertshRNA targeting PHB (see partial depositor seq) (PHB1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
ROCK2 gRNA (BRDN0001145177)
Plasmid#76351Purpose3rd generation lentiviral gRNA plasmid targeting human ROCK2DepositorInsertROCK2 (ROCK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-H1-SGINX
Plasmid#174844Purposeempty vector for cloning shRNA and/or miRNADepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-Rap2A-3xFLAG
Plasmid#87974Purpose3rd generation lentiviral vector for Expression of Rap2A in mammalian cellsDepositorInsertRAP2A (RAP2A Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001148372)
Plasmid#80258Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
CLTC G10.4 gRNA
Plasmid#90635Purpose3rd generation lentiviral gRNA plasmid targeting human CLTCDepositorInsertCLTC (Guide Designation G10.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgNeg2
Plasmid#166999PurposeLentiviral expression of negative control sgRNA for CRISPRi knockdownDepositorInsertNegative control sgRNA 2
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (CCR5 TALEN Sce Spacer)
Plasmid#46941PurposeCodes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with TALEN TS
+3 mCherry
3' iRFP arm
UseLentiviralTagsExpressionBacterial and MammalianMutationembedded TALEN TS from 163-218, truncated 25 amin…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
PWPXLD-GFP-Numb-S276D
Plasmid#133752PurposeNumb with mutation at serine 276 converted to aspartic acid cand tagged with GFP cloned into PWPXLDDepositorInsertNUMB endocytic adaptor protein (Numb Mouse)
UseLentiviralTagsGFPExpressionMammalianMutationserine 276 converted to aspartic acidPromoterEF1-alphaAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
sh-RNA hULK2
Plasmid#31970DepositorInsertULK2 (ULK2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only