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Plasmid#114223PurposePOC12359, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type d-eDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLC_aFq
Plasmid#114224PurposePOC12362, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type a-qDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLC_bFq
Plasmid#114225PurposePOC12363, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type b-qDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLC_cFq
Plasmid#114226PurposePOC12364, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type c-qDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLC_dFq
Plasmid#114227PurposePOC12365, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type d-qDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXLC_eFq
Plasmid#114228PurposePOC12366, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type e-qDepositorInsertLacZalpha
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBHDuet50K
Plasmid#118399Purposeanalysis of cis-splicing by mini inteinsDepositorInsertMvuTFIIB mini intein
UseTagsExpressionBacterialMutationincorporation of oligonucleotide during cloningPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMPO 51
Plasmid#68358PurposeExpression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2). ApR, expression vector with rrnBT1T2-Pm-MCSII, ColE1 replication originDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
DHRS11
Plasmid#39157PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDHRS11 (DHRS11 Human)
UseTagsHis TevExpressionBacterialMutationG99L100 -> AHM; codon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTP396
Plasmid#149336PurposeBacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobodyDepositorInsertanti-GFP nanobody
UseTagsBiotin acceptor peptide (Avi-tag), His14, and SUM…ExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA2
Plasmid#85710PurposeThis plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomesDepositorTypeEmpty backboneUseCre/Lox; Herpesvirus cloningTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_3XFlag
Plasmid#182494PurposeEmpty plasmid containing Flag tag for C-terminal taggingDepositorInsert3xFlag with 5 Glycolic spacer
UseTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP wild type
Plasmid#174075PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoterDepositorInsertSuperfolder GFP
UseTagsHis-6ExpressionBacterialMutationPromoterT7Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-TurboID-NES_pLX304
Plasmid#202008Purposeexpresses TurboID in the mammalian cytosol, lentiviral vectorDepositorInsertFlag-TurboID-NES
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Alive
Plasmid#20860PurposeAlive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.DepositorInsertStreptavidin-Alive
UseTagsHisExpressionBacterialMutationWt genePromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Dead
Plasmid#20859PurposeDead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).DepositorInsertStreptavidin-Dead
UseTagsExpressionBacterialMutationTriple mutant of wt Streptavidin N23A,S27D,S45APromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
V5-GFP-APEX2-NES_pLX208
Plasmid#202018Purposeexpresses GFP-APEX2 in the mammalian cytosol, lentiviral vectorDepositorInsertV5-GFP-APEX2-NES
UseLentiviralTagsGFP and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
V5-GFP-TurboID-KDEL_pLX208
Plasmid#202020Purposeexpresses GFP-TurboID in the mammalian ER lumen, lentiviral vectorDepositorInsertV5-TurboID-KDEL
UseLentiviralTagsGFP and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CMV::V5-EGFR
Plasmid#199717PurposeExpresses V5-tagged EGFR in mammalian cells.DepositorInsertV5-EGFR
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CMV::hTfR-GFP
Plasmid#199714PurposeExpresses a chimera of human transferrin receptor (hTfR) and GFP in mammalian cells.DepositorInserthTfR-GFP
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only