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AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97308

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

AAV and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-7xTRE-3xeGFP

Plasmid

#191206

Purpose

GFP expression under the control of TRE promotor

Depositor

Janelia Viral Tools

Article

Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

Insert

3X GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TetON-CD4

Plasmid

#217537

Purpose

AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged CD4 receptor

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

CD4 receptor (CD4 Human)

Use

AAV

Tags

HA epitope

Expression

Mammalian

Mutation

Promoter

TetOperon (TetO)

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-FlicR1

Plasmid

#74143

Purpose

Express FlicR1 preferentially in neurons

Depositor

Robert Campbell

Article

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Insert

FlicR1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV GFAP jGCaMP7b

Plasmid

#171118

Purpose

Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter

Depositor

Thomas Oertner

Article

Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

Insert

jGCaMP7b

Use

AAV

Tags

6 His Tag, T7 Tag, and Xpress Tag

Expression

Mammalian

Mutation

Promoter

GFAP

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-AAVS1-sg

Plasmid

#194716

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hAAVS1 locus

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1 (AAVS1 Acidaminococcus sp.)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-ABE-NT-sgRNA

Plasmid

#112734

Purpose

AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA

Depositor

Jin-Soo Kim

Article

Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

Insert

ABE-NT-sgRNA

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-DCX-2A-mCherry

Plasmid

#118290

Purpose

Expresses human DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.

Depositor

Vance Lemmon

Article

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

doublecortin (DCX Human)

Use

AAV

Tags

2A peptide and mCherry

Expression

Mammalian

Mutation

Promoter

CMV with beta global intron

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.FLEX.iGluSnFR3.v857.PDGFR

Plasmid

#196219

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in PDGFR backbone. iGluSnFR3.v857.PDGFR

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV CAG FLEX iGluSnFR3 v857.PDGFR

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and PDGFR TM Domain

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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HT116_pAAV_hSyn-DiO-SomQuasAr6b_EGFP

Plasmid

#190879

Purpose

Cre-on expression of soma-targeted QuasAr6b under an neuronal promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

soma-targeted QuasAr6b

Use

AAV

Tags

EGFP

Expression

Mutation

Promoter

hSyn

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FRISZ-TM

Plasmid

#176886

Purpose

AAV transfer plasmid for cell-surface-localized FRISZ (via PDGFR TM) under hSyn promoter

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

Ig kappa-FRISZ-PDGFR TM

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Sa-SauriCas9

Plasmid

#135967

Purpose

Expresses Sa-SauriCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-SN

Plasmid

#181741

Purpose

AAV vector for expressing SN

Depositor

Yasunori Hayashi

Article

Goto et al Science. 2021 Nov 12;374(6569):857-863.

Insert

SuperNova (monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-AAVS1_sgRNA

Plasmid

#183891

Purpose

pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FDIO-dAPEX2

Plasmid

#117175

Purpose

Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

dAPEX2

Use

AAV; Flp/frt

Tags

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97311

Purpose

MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb MMEJ donor

Use

AAV and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-GFAP-mNeonGreen

Plasmid

#105713

Purpose

AAV packaging vector to express mNeonGreen in astrocytes.

Depositor

Pantelis Tsoulfas

Insert

mNeonGreen

Use

AAV

Tags

Expression

Mutation

Promoter

Minimal GFAP promoter

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-rMERTK-HITI

Plasmid

#87119

Purpose

Gene correction donor AAV for RCS rat. AAV backbone plasmid including exon 2 of rat Mertk and rMertkgRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-rMertksgRNA-Mertk(intron1-2)-nEF-GFPKASH-pA

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_NPYmut

Plasmid

#208679

Purpose

Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV- CAG-h2BmRuby2FLAG

Plasmid

#116872

Purpose

Nuclear localized mRuby2FLAG

Depositor

Loren Looger

Article

Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

Insert

H2BmRuby2FLAG

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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