1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pKLV2-EF1a-GFP ArgSen

   Plasmid

   #184695

   Purpose

   Lentiviral transfer plasmid for expression of GFP ArgSen

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   GFP ArgSen
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CAG-loxP-3xpA-loxP-H2B-sfGFP

   Plasmid

   #136064

   Purpose

   H2B-sfGFP cre reporter

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   HIST1H2BB (H2BC3 Human)
   

   Use

   Cre/Lox

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. YPRCΔ15Ω1

   Plasmid

   #101710

   Purpose

   GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Type

   Empty backbone

   Use

   Yeast expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUB6-VSV-GS

   Plasmid

   #190781

   Purpose

   Expresses VSV-GS in mammalian cells

   Depositor

   Jakob Reiser

   Article

   Zhang et al Retrovirology. 2010 Jan 25;7:3. doi: 10.

   Insert

   VSV-GS
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ePPE-V223A

   Plasmid

   #205243

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   SV40_NLS-nCas9-NC-MLV-SV40_NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Detailed in manuscript

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBBK

   Plasmid

   #62670

   Purpose

   Vector for PhiC31o mRNA synthesis

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOPTN217-577-EGFP

   Plasmid

   #68841

   Purpose

   deleted OPTN was inserted into pEGFP-N1 backbone

   Depositor

   Beatrice Yue

   Article

   Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

   Insert

   optineurin (OPTN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 210-577 only

   Promoter

   CMV

   Available since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pOPTN1-209-EGFP

   Plasmid

   #68839

   Purpose

   deleted OPTN was inserted into pEGFP-N1 backbon

   Depositor

   Beatrice Yue

   Article

   Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

   Insert

   optineurin (OPTN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-209 only

   Promoter

   CMV

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

9. HA-Cer-NCreIntN

   Plasmid

   #160505

   Purpose

   Expresses NCreIntN, tagged with HA, in mammalian cells in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   NCreIntN
   

   Use

   Cre/Lox

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEU3-NII-FLAGLICNot

    Plasmid

    #140184

    Purpose

    Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, FLAG-tag and NotI restriction site

    Depositor

    Tamás Mészáros

    Article

    Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

    Type

    Empty backbone

    Use

    Expression vector for a cell-free system

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDD317 (Pmyo-2::GFP + SEC)

    Plasmid

    #73344

    Purpose

    SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

    Insert

    Pmyo-2::GFP + SEC
    

    Use

    CRISPR and Cre/Lox

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    myo-2

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSF279

    Plasmid

    #167674

    Purpose

    Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQ398X-EGFP

    Plasmid

    #68849

    Purpose

    mutated OPTN was inserted into pEGFP-N1 backbone

    Depositor

    Beatrice Yue

    Article

    Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q398X, results in truncation of the protein into …

    Promoter

    CMV

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pRU325

    Plasmid

    #167676

    Purpose

    Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRU323

    Plasmid

    #167690

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pICH47742:FCP:Cas9YFP

    Plasmid

    #85986

    Purpose

    Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR; Golden gate assembly

    Tags

    SV40 NLS, YFP

    Expression

    Mutation

    Promoter

    FCP

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-PxCas12a-2AeGFP

    Plasmid

    #123637

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    PxCas12a
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDGE1109

    Plasmid

    #202773

    Purpose

    CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:TREX2-zCas9_ttriple
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FLAG-IntCCCre

    Plasmid

    #160506

    Purpose

    Expresses IntCCCre, tagged with FLAG, in mammalian cells in split intern-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRU319

    Plasmid

    #167686

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge