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Plasmid#24909DepositorInsertfishRab8B
UseGateway entry vectorTagsExpressionMutationChanged Threonine 22 to AsparaginePromoterAvailable sinceJune 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSM045
Plasmid#217399PurposeKmR, pBTRcK broad host range vector with BTE1(C12-specific fatty acid thioesterase codon-optimised for C. necator) under PtrcDepositorInsertBTE1
UseTagsExpressionBacterialMutationPromoterPTrcAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSM046
Plasmid#217400PurposeKmR, pBTRcK broad host range vector with BTE2(C12-specific fatty acid thioesterase codon-optimised for C. necator) under PtrcDepositorInsertBTE2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTrcAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tc-INTR-GFP
Plasmid#208899Purposeencodes the inotocin receptor (oxytocin/vasopressin-like) from the beetle Tribolium castaneum, tagged with GFPDepositorInsertINTR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AP-VAMP1(X)
Plasmid#167999Purposepositive selection PACE Accessory PlasmidDepositorInsertVAMP1(L56-L72)-T7PAP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
AP-Ykt6
Plasmid#167997Purposepositive selection PACE Accessory PlasmidDepositorInsertYkt6(L161-L177)-T7PAP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
AP-VAMP5
Plasmid#168001Purposepositive selection PACE Accessory PlasmidDepositorInsertVAMP5(L28-L44)-T7PAP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHis8-4b-McDAH
Plasmid#140129PurposeExpresses McDAH with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase from Menispermum canadense
UseTags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialMutationPromoterT7Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNB2629
Plasmid#113394PurposeMET25p-MS2-RFP-CYC1 TTDepositorInsertMS2 bacteriophage coat protein
UseTagsred fluorescent protein (RFP), codon-optimized fo…ExpressionYeastMutationPromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBALU3
Plasmid#69318PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of cfpDepositorInsertCFPint-FgF
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBALU2
Plasmid#69317PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of yfpDepositorInsertYFPint-FgF
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
PCx35-pcDNA-S110A
Plasmid#42917DepositorInsertConnexin 35
UseTagsExpressionMammalianMutationSer110 to AlaPromoterCMVAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
PCx35CT-S298ter
Plasmid#42913DepositorInsertConnexin 35 C-terminal domain
UseTags6x His, GST, and S-tagExpressionBacterialMutationSer298 stop codonPromoterT7Available sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
PCx35-pcDNA-S298ter
Plasmid#42925DepositorInsertConnexin 35
UseTagsExpressionMammalianMutationSer298 to stop codonPromoterCMVAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
PCx35CT-S276A
Plasmid#42912DepositorInsertConnexin 35 C-terminal domain
UseTags6x His, GST, and S-tagExpressionBacterialMutationSer276 to AlaPromoterT7Available sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-rRNA-5'ETS 923 to 1179
Plasmid#23995DepositorInsertrRNA 5'ETS ntds 923 to 1179
UseBacterial cloning - topoTagsExpressionMutationNonePromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-rRNA-5'ETS 1 to 177
Plasmid#23993DepositorInsertrDNA 5'ETS ntds 1 to 177
UseBacterial cloning - topoTagsExpressionMutationNonePromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-25S rDNA
Plasmid#23996DepositorInsert25S rRNA from +5270 to oligo y
UseBacterial cloning - topoTagsExpressionMutationnonePromoterAvailable sinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B-NCre
Plasmid#106368PurposeN-terminal part of the split-Cre SystemDepositorInsertNCre
UseCre/LoxTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
NLRP3-mNeonGreen
Plasmid#200942PurposeNLRP3 fused with mNeonGreen to indicate its localization changeDepositorInsertNLRP3 (NLRP3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.blast-Renilla_Luciferase
Plasmid#74444PurposeControl lentiviral vector expressing Renilla luciferaseDepositorInsertRenilla Luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
ClCry4 PHR
Plasmid#160552PurposeExpression of protein for various experiments including finding interaction partnersDepositorInsertClcry4 HR
UseTagsHexa hisExpressionInsectMutationPromoterAvailable sinceNov. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag3B-CCre
Plasmid#106369PurposeC-terminal part of the split-Cre SystemDepositorInsertCCre
UseCre/LoxTagsc-mycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro Xlone-eGFP
Plasmid#136936Purposedonor plasmid for inducible transgene expression in human cellsDepositorInsertall-in-one tet-on system
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV4.50-mut
Plasmid#201059PurposeExpresses AAV2 Rep and AAV4.50 CapDepositorInsertAAV4.50 Cap
UseAAVTagsExpressionMammalianMutationN586R, S587A, N588L, L589RPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-X(5010)B1
Plasmid#168019PurposeExpresses X(5010)B1-6xHis in bacterial cellsDepositorInsertX(5010)B1-6xHis
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-mCherry
Plasmid#114013PurposeEmpty sgRNA expression vector with co-expression of mCherry reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-GFP
Plasmid#114012PurposeEmpty sgRNA expression vector with co-expression of TurboGFP reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-X(4130)B1
Plasmid#168018PurposeExpresses X(4130)B1-6xHis in bacterial cellsDepositorInsertX(4130)B1-6xHis
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-CV-SNAP25
Plasmid#168031PurposeExpresses eCFP-SNAP25(A141-G206)-mVenus in bacterial cellsDepositorInserteCFP-SNAP25(A141-G206)-mVenus
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only