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pECFP-C1-rGAT

Plasmid

#70108

Purpose

mammalian expression of rat GAT with N terminal CFP fusion

Depositor

Harald Sitte

Article

Scholze et al J Biol Chem. 2002 Nov 15;277(46):43682-9

Insert

GAT (Slc6a1 Rat)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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EMSV-MRF4 (puro)

Plasmid

#14713

Depositor

Michael Rudnicki

Article

Sabourin et al Clin Genet. 2000 Jan . 57(1):16-25.

Insert

MRF4 (Myf6 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2007

Availability

Academic Institutions and Nonprofits only
pCS2-LAMP1-EGFP

Plasmid

#169003

Purpose

Express rat LAMP1-EGFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Cell Rep. 2020 Dec 8;33(10):108477. doi:

Insert

rat LAMP1-EGFP

Use

Unspecified

Tags

EGFP

Expression

Mutation

Promoter

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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Igfbp1-promoter/pGL3

Plasmid

#12146

Depositor

Domenico Accili

Article

Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

Insert

IGFBP1 promoter (Igfbp1 Rat)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains the canonical insulin response sequence …

Promoter

Available since

Aug. 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-AU1-mTOR-R2505P

Plasmid

#26038

Depositor

Fuyuhiko Tamanoi

Article

Sato et al Oncogene. 2010 May 6. 29(18):2746-52.

Insert

mammalian target of rapamycin-R2505P (Mtor Rat)

Use

Tags

AU1

Expression

Mammalian

Mutation

R2505P

Promoter

Available since

Sept. 21, 2010

Availability

Academic Institutions and Nonprofits only
pOTTC946 - pAAV CMV-IE NK1R-IRES-EGFP

Plasmid

#102935

Purpose

An AAV vector that expresses rat NK1R-IRES-EGFP under the CMV-IE promoter

Depositor

Brandon Harvey

Article

Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

Insert

NK1R (Tacr1 Rat)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSIN ReP5-GFP-GluR1

Plasmid

#24004

Depositor

Robert Malinow

Article

Kessels et al Nat Neurosci. 2009 Jul . 12(7):888-96.

Insert

GluR1 (Gria1 Rat)

Use

Sindbis virus production

Tags

GFP

Expression

Mutation

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only
pLV302

Plasmid

#119943

Purpose

Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

Depositor

Gerald Schwank

Article

Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

Insert

N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-evoA1-seBEN_empty

Plasmid

#174701

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

Lenti-evoA1-seBEN

Use

Lentiviral

Tags

myc tag

Expression

Mutation

Promoter

EFS/U6

Available since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-evoA1-seBEC

Plasmid

#174702

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

Lenti-evoA1-seBEC

Use

Lentiviral

Tags

flag tag

Expression

Mutation

UGI-E11K

Promoter

EFS

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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cacnb1b (rat) in pMT2 vector

Plasmid

#107423

Purpose

Expresses Calcium channel beta1b auxillary subunit in mammalian cells.

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 1997 Feb 15;17(4):1330-8.

Insert

calcium channel beta1b auxillary subunit

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ad MLP

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-MyosinVb-Ctail

Plasmid

#110170

Purpose

Dominant negative effect by competing with MyosinVb C-terminal tail interactions

Depositor

Jose Esteban

Article

Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

Insert

Myosin Vb (Myo5b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

amino acids 1262-1670

Promoter

CMV

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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N-WASP (WGP)

Plasmid

#11597

Depositor

Marc Kirschner

Article

Rohatgi et al J Cell Biol. 2000 Sep 18. 150(6):1299-31

Insert

N-WASP (Iqub Rat)

Use

Tags

Expression

Mammalian

Mutation

contains amino acids 1-396

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pOC2 CRISPIE-Halo-Homer1 KI

Plasmid

#183446

Purpose

CreON knock-in for Halo-Homer1 (amino acid position: G2)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and CRISPIE Halo donor

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Nrxn1α-LNS2

Plasmid

#128106

Purpose

Express HA tagged Nrxn1α-LNS2

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nrxn1a-LNS2 (Nrxn1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8-Magneto2.0

Plasmid

#74333

Purpose

For gateway cloning Magneto2.0 into destination vector

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

Magneto2.0

Use

Gateway vector

Tags

Flag

Expression

Mutation

TRPV4: delta 760-871

Promoter

none

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-sACt-mCherry

Plasmid

#138216

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

Lyn-sACt-mCherry (Adcy10 Rat)

Use

Tags

N-terminal targeting sequence from Lyn kinase and…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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TSC2-3A (S1337A/S1341A/S1345A)

Plasmid

#29360

Depositor

Kunliang Guan

Article

Inoki et al Cell. 2003 Nov 26. 115(5):577-90.

Insert

TSC2 (Tsc2 Rat)

Use

Tags

Expression

Mammalian

Mutation

S1337A/S1341A/S1345A (equivalent to S1381/S1385/S…

Promoter

Available since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only
CD200Cd4d3+4-bio

Plasmid

#32404

Purpose

A positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200

Use

Tags

Cd4d3+4 and biotinylation peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pEF Doc2alpha WT GFP

Plasmid

#128819

Purpose

To visualize Doc2alpha WT in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2alpha (Doc2a Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1alpha

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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