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pAAV_-CMV-Pcdh15_mini_V4-CD2

Plasmid

#199184

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

mmPcdh15_mini_V4-CD2

Use

AAV

Tags

Expression

Mutation

EC4, EC5, EC6, EC7, EC8 are deleted from msPCDH15…

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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RCAS(B) mScx (CT#1035)

Plasmid

#13899

Depositor

Cliff Tabin

Article

Brent et al Cell. 2003 Apr 18. 113(2):235-48.

Insert

Scleraxis (Scx Mouse)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
PLIN2-V5-APEX2

Plasmid

#170573

Purpose

A C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2

Depositor

James Olzmann

Article

Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

Insert

PLIN2 (Plin2 Mouse)

Use

Lentiviral

Tags

V5-APEX2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG mFoxg1-GFP wildtype

Plasmid

#73048

Purpose

C-terminal fusion of gfp Mouse wildtype Foxg1

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

mFoxg1 (Foxg1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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mPlum-LC-Myosin-N-7

Plasmid

#55983

Purpose

Localization: Light Chain Myosin, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Insert

LC-Myosin (Myl9 Mouse)

Use

Tags

mPlum

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pBABE puro mouse IRS-2 myc

Plasmid

#11373

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-2 (Irs2 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pLV-puro-N-3HA-SREBP2-C-Flag

Plasmid

#134287

Purpose

Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP2 (Srebf2 Mouse)

Use

Lentiviral

Tags

3x HA and Flag

Expression

Mammalian

Mutation

mouse SREBP2 precursor

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21-Q76L (CA)

Plasmid

#83422

Purpose

Mouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Residue 76 from Q to L (Q76L: CAG -> CTG)

Promoter

CMV

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GATA1

Plasmid

#85693

Purpose

Mammalian expression of mouse GATA1

Depositor

Giannino Del Sal, Licio Collavin

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

GATA1 (Gata1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-mGCN2-FLAG-IP

Plasmid

#101794

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse GCN2

Depositor

Seiichi Oyadomari

Article

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Gcn2 (Eif2ak4 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-mC/EBPb

Plasmid

#49198

Purpose

expression of mouse C/EBPbeta in mammalian cells

Depositor

Jed Friedman

Insert

C/EBPbeta (Cebpb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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Px461-Cas9n-Trp53-sgRNA-beta

Plasmid

#88847

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
pISH-Adnp

Plasmid

#74340

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Adnp. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

activity-dependent neuroprotective protein (Adnp Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRetroX-mCRT-GPI-IRES-DsRed

Plasmid

#223711

Purpose

Mammalian expression of mouse CRT-GPI

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

Calreticulin-GPI (Calr Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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PDGFRalpha-D842V #1

Plasmid

#201407

Purpose

Inserts PDGFRalpha-D842V to activate the MAP kinase pathway.

Depositor

Maria Castro

Article

Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

Insert

PDGFRalpha-D842V (Pdgfra Mouse)

Use

Tags

Expression

Mammalian

Mutation

D842V

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_mmIrf1

Plasmid

#160098

Purpose

Expresses murine Irf1 in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Irf1 (Irf1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

Plasmid

#194284

Purpose

EF1a-dCas9-KRAB-GFP with nontarget sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mutation

Promoter

EF1a

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pmNFH

Plasmid

#85178

Purpose

Mouse neurofilament protein H cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Colakoglu et al J Cell Biol. 2009 Jun 1;185(5):769-77. d

Insert

mouse NFH (Nefh Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pJFRC2-10XUAS-IVS-mCD8::GFP

Plasmid

#26214

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Aug 9. ():.

Insert

mCD8::GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-GST-eIF4E K119A

Plasmid

#112818

Purpose

Expresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Eif4e (Eif4e Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

K119 mutated to A, increasing affinity for cap st…

Promoter

T7

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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