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pAS3-npr-9P::AI::lgc-47::SL2::tagBFP::unc-54-3'UTR

Plasmid

#225923

Purpose

Expressing LGC-47 in AIB neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

npr-9

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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MDH2_EC

Plasmid

#224146

Purpose

IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.

Depositor

Katherine Walstrom

Article

Thomas et al Biochim Biophys Acta Proteins Proteom. 2

Insert

MDH-2 (mdh-2 Nematode)

Use

Tags

His tag, Xpress tag, enterokinase cleaveage site

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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GPD1_SB

Plasmid

#224147

Purpose

IPTG-inducible expression of C. elegans protein GPD-1 with a chitin tag

Depositor

Katherine Walstrom

Insert

GPD-1 (gpd-1 Nematode)

Use

Tags

Ala-intein-CBD

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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ADH1_KW

Plasmid

#224149

Purpose

IPTG-inducible expression of C. elegans protein ADH-1 with a chitin tag

Depositor

Katherine Walstrom

Insert

ADH-1 (sodh-1 Nematode)

Use

Tags

Ala-intein-CBD

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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GPD3_KW

Plasmid

#224151

Purpose

IPTG-inducible expression of C. elegans protein GPD-3 with a chitin tag

Depositor

Katherine Walstrom

Insert

GPD-3 (gpd-3 Nematode)

Use

Tags

intein-CBD

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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ALDO-1_KS

Plasmid

#224157

Purpose

IPTG-inducible expression of C. elegans protein ALDO-1 with a His tag

Depositor

Katherine Walstrom

Insert

ALDO-1

Use

Tags

LeuGlu-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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MDH1_KW

Plasmid

#224145

Purpose

IPTG-inducible expression of C. elegans protein MDH-1 with a chitin tag

Depositor

Katherine Walstrom

Article

Thomas et al Biochim Biophys Acta Proteins Proteom. 2

Insert

MDH-1 (F46E10.10 Nematode)

Use

Tags

Ala-intein-CBD

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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GSPD1_R252L

Plasmid

#224153

Purpose

IPTG-inducible expression of C. elegans protein GSPD-1(R252L) with a His tag

Depositor

Katherine Walstrom

Article

Loges et al MicroPubl Biol. 2021 Sep 10;2021:10.1791

Insert

GSPD-1(R252L)

Use

Tags

LeuGlu-6xHis

Expression

Bacterial

Mutation

R252L

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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GSPD1_AJ

Plasmid

#224152

Purpose

IPTG-inducible expression of C. elegans protein GSPD-1 with a His tag

Depositor

Katherine Walstrom

Article

Loges et al MicroPubl Biol. 2021 Sep 10;2021:10.1791

Insert

GSPD-1 (B0035.5 Nematode)

Use

Tags

LeuGlu-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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GSPD1_D60N

Plasmid

#224154

Purpose

IPTG-inducible expression of C. elegans protein GSPD-1(D60N) with a His tag

Depositor

Katherine Walstrom

Article

Loges et al MicroPubl Biol. 2021 Sep 10;2021:10.1791

Insert

GSPD-1(D60N)

Use

Tags

LeuGlu-6xHis

Expression

Bacterial

Mutation

D60N

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY142

Plasmid

#223489

Purpose

unc-17(3k) fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-17(3k) (unc-17 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY136

Plasmid

#223483

Purpose

tag-168 fluorescent neural reporter driving nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tag-168

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY120

Plasmid

#223467

Purpose

sra-11(pB) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

sra-11(pB) (sra-11 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY118

Plasmid

#223465

Purpose

ser-2(prom3) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ser-2(prom3) (ser-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY114

Plasmid

#223461

Purpose

pdf-1 fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

pdf-1 (pdf-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEY110

Plasmid

#223457

Purpose

odr-10 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

odr-10 (odr-10 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY100

Plasmid

#223447

Purpose

lad-2 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

lad-2 (lad-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY76

Plasmid

#223445

Purpose

dop-2 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

dop-2 (dop-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEY74

Plasmid

#223444

Purpose

cho-1(3.1-2.6k) fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

cho-1(3.1-2.6k) (cho-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY139

Plasmid

#223486

Purpose

tph-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tph-1 (tph-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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