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pSNAPf-H2B Control Plasmid

Plasmid

#101124

Purpose

Expression of SNAP-tagged Histone H2B in mammalian cells

Depositor

Ana Egana, New England Biolabs

Insert

histone cluster 1 H2B family member j (H2BC11 Human)

Use

Tags

SNAP-tag (SNAPf)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCW-Cas9

Plasmid

#50661

Purpose

Doxycycline-inducible lentiviral expression of SpCas9

Depositor

David Sabatini, Eric Lander

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Tet ON

Available since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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v5 ABE-eVLP

Plasmid

#228467

Purpose

Expresses MMLVgag(C507V)–ABE8e for producing v5 ABE-eVLPs

Depositor

David Liu

Article

Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

Insert

MMLVgag(C507V)–ABE8e

Use

Tags

FLAG

Expression

Mammalian

Mutation

MMLVgag(C507V)

Promoter

CMV

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pOpen-MMLV_RT (lack H)

Plasmid

#165556

Purpose

Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.

Depositor

Jennifer Molloy, Drew Endy, FreeGenes Project

Insert

Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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eGFP-KIF5B

Plasmid

#172203

Purpose

Expresses KIF5B tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF5B (Kif5b Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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C1(1-29)-TurboID-V5_pCDNA3

Plasmid

#107173

Purpose

expresses V5-tagged TurboID on the mammalian ER membrane

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Use

Tags

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RIPK1

Plasmid

#78842

Purpose

To overexpress RIPK1 in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pOTTC476 - pAAV c-fos eYFP

Plasmid

#47907

Purpose

An AAV vector that expresses eYFP under the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

enhanced Yellow Fluorescent Protein

Use

AAV

Tags

Expression

Mutation

Promoter

c-fos

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)

Plasmid

#149276

Purpose

Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence

Use

T-dna vector

Tags

Expression

Mutation

T2318C (DNA) in PEBV coat protein sequence

Promoter

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEvol-pAzFRS.2.t1

Plasmid

#73546

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.2.t1
pAzFRS.2.t1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPA1A

Plasmid

#19456

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPA1A (HSPA1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagB wt

Plasmid

#19313

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pINDUCER21 (ORF-EG)

Plasmid

#46948

Purpose

Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

Depositor

Stephen Elledge, Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

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PX458-AAVS1

Plasmid

#113194

Purpose

Encodes gRNA for human AAVS1

Depositor

Adam Karpf

Insert

AAVS1 (PPP1R12C, AAVS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLX302 Luciferase-V5 puro

Plasmid

#47553

Purpose

Lentiviral expression of V5-tagged Luciferase in mammalian cells

Depositor

Kevin Janes

Article

Kang et al Sci Signal. 2013 Aug 6;6(287):rs13. doi:

Insert

Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pWUR790

Plasmid

#101723

Purpose

Synthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgo

Depositor

John van der Oost

Article

Swarts et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

PfAgo

Use

Tags

Strep(II)-tag

Expression

Bacterial

Mutation

Promoter

T7 Promotor

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDonor_ShCAST_kanR

Plasmid

#127924

Purpose

Kanamycin resistance donor for ShCAST

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

KanR, R6K origin of replication

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEB3-mScarlet3_N1

Plasmid

#189770

Purpose

Construct for labelling microtubule plus ends in mammalian cells with EB3-mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

EB3-mScarlet3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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MP6

Plasmid

#69669

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR ugi CDA1

Use

Tags

Expression

Bacterial

Mutation

Promoter

E. coli PBAD

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-PV-NES-GFP

Plasmid

#17301

Depositor

Anton Bennett

Article

Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

Insert

PV-NES-GFP (Pvalb Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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