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Plasmid#50708Purposeexpresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTsDepositorInsertEB3 (MAPRE3 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUMVC
Plasmid#8449PurposePackaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2005AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-MCP-HALOnls
Plasmid#121937PurposeCRISPR-Sirius plasmidDepositorInsertMCP-HALOnls
UseLentiviralTagsHaloTagExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 APEX2-NES
Plasmid#49386PurposeExpresses APEX2 in the cytosol of mammalian cellsDepositorInsertFlag - APEX2 - NES
UseTagsFlagExpressionMammalianMutationK14D, W41F, E112K, A134P on soybean APX; improved…PromoterCMVAvailable sinceNov. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody
Plasmid#199390PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tagDepositorInsertanti-ALFA nanobody
UseTags14xHis-Avi-SUMOEuExpressionBacterialMutationPromoterT5-LacOAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
COM-epegRNA-Dnmt1
Plasmid#211376PurposeDnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP productionDepositorInsertCOM-epegRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human V5-IRF3-pcDNA3
Plasmid#32713DepositorInsertIRF3 (IRF3 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
Lck-mScarlet-I
Plasmid#98821PurposeIn vivo visualization of the plasma membrane (can be used for colocalization studies)DepositorArticleInsertLck
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRia-v2
Plasmid#84832PurposeCRISPRi/a V2 library parental plasmidDepositorInsertssgRNA
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-recX-sacB
Plasmid#158718PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.DepositorInsertrecX
UseTagsExpressionBacterialMutationPromoterPmycAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMBIS
Plasmid#17817DepositorInsertmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-EGFP-Tau P301L
Plasmid#46908Purposeexpresses EGFP tagged Tau P301L in mammalian cellsDepositorInsertTau P301L (MAPT Human)
UseTagsEGFPExpressionMammalianMutationP301LPromoterCMVAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
MS2-epegRNA-Dnmt1
Plasmid#211372PurposeDnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP productionDepositorInsertMS2-epegRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_550
Plasmid#203398PurposeAll-in-one lentiviral expression of gRNA and EnAsCas12aDepositorInsertEnhanced Acidaminococcus Cas12a
UseCRISPR and LentiviralTagsExpressionMutationPromoterEF1aAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mGFPmut2
Plasmid#103980PurposePlasmid encoding mGFPmut2DepositorInsertmGFPmut2
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S65A, V68L, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJR98
Plasmid#187239PurposeCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sitesDepositorInsertCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-puro-cOVA-IRES-BFP
Plasmid#135074PurposeExpresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.DepositorInsertcOVA-IRES-BFP (OVAL Chicken)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
Plasmid#60903PurposeExpressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activationDepositorInsertCas9 dead
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTNS2
Plasmid#64968PurposeTn7 transposase expressionDepositorInserttnsABCD
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits