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Plasmid#105951Purposeheat shock inducible transgenesisDepositorInsertRas
UseTagsmKate2ExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-hOPTN-D474N
Plasmid#23055DepositorInsertOptineurin (OPTN Human)
UseTagsHisExpressionBacterialMutationD474NPromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP mouse Rab35 DN
Plasmid#46016DepositorInsertRab35 (Rab35 Mouse)
UseDrosophilaTagsYFPExpressionInsectMutationS22N (dominant negative)PromoterGAL4Available sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXL-CAG-mBirA272-NRX3b D137A
Plasmid#43922DepositorInsertBirA272-NRX3b (birA Synthetic, Human)
UseTags2XFLAGExpressionMammalianMutationD137APromoterCAGAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pME-6MTzactMap2k2b
Plasmid#67710PurposeMiddle entry cloneDepositorInsertactivated Map2k2b (map2k2b Zebrafish)
UseTagsExpressionMutationS221D, S225DPromoternoneAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
LIIc F 1-2 RNAi
Plasmid#54402DepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST myc mouse Rab35 CA
Plasmid#53516DepositorInsertRab35 (Rab35 Mouse)
UseTagsmycExpressionInsectMutationQ67LPromoterGAL4Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-hOPTN-D474N
Plasmid#23054DepositorInsertoptineurin (OPTN Human)
UseTagsGSTExpressionBacterialMutationD474NPromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDD401
Plasmid#91828PurposePmyo-2::GFP FP-slot donor for the SapTrap cloning systemDepositorInsertPmyo-2::GFP
UseCRISPRTagsExpressionWormMutationNucleotide 489 of the myo-2 promoter was changed …PromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CYP2A13
Plasmid#67103PurposeStandard for cell-free expression benchmarking.DepositorInsertCYP2A13 (CYP2A13 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pExp_TRE:TAL14
Plasmid#51794PurposeInducible expression of TAL14 as input repressorDepositorInsertTAL14
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTREAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMB-PLAr
Plasmid#61201PurposeFluorescent reporter for plastids MtRubisco1sp-mCherry expressed under MtBCP1 promoterDepositorInsertMtRubisco1sp-mCherry
UseTagsmCherryExpressionPlantMutationPromoterMtBCP1Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEG608
Plasmid#40194DepositorInsertMex-5 (RNAi resistant) (mex-5 Nematode)
UseTags3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…ExpressionWormMutationExon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFLIP30
Plasmid#65499PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pInducer21 Flag-Sesn3
Plasmid#61866PurposeDox inducible lentivirial expression of Flag-Sesn3 (mouse)DepositorInsertSesn3 (Sesn3 Mouse)
UseTagsFlagExpressionMammalianMutationnonePromoterAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFDP2
Plasmid#67088PurposeStandard for cell-free expression benchmarking.DepositorInsertTFDP2 (TFDP2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP32
Plasmid#65500PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP39
Plasmid#65507PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pExp_TRE:TAL21
Plasmid#51795PurposeInducible expression of TAL21 as input repressorDepositorInsertTAL21
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTREAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAST tdTomato Rab35 DN
Plasmid#53499DepositorInsertRab35 (Rab35 Fly)
UseTagstdtomatoExpressionInsectMutationS22NPromoterGAL4Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only