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Plasmid#167750PurposeExpresses ELK3 in mammalian cellsDepositorInsertELK3 (ELK3 Human)
UseTagsMACExpressionMammalianMutationno mutationsPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6-LRRC52-V5-Flag-His
Plasmid#127818PurposeExpression of human LRRC52 in mammalian cellsDepositorInsertLRRC52 (LRRC52 Human)
UseTagsV5, FLAG, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2Apuro-TRE-synPCB2.0
Plasmid#139481PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
UseTol2 transposon donor vectorTagsExpressionMammalianMutationPromoterTet responsive elementAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-TAPP1(PHD)
Plasmid#221757PurposeStable expression of GFP-TAPP1(PHD) in mammalian cellsDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem TAPP1 (PHD) (amino acids 184-304)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
NPFF2-DuET
Plasmid#213352PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertNPFF2 (NPFFR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
NPBW2-DuET
Plasmid#213351PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertNPBW2 (NPBWR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSTR2-DuET
Plasmid#213377PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertSSTR2 (SSTR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-mSox2C17
Plasmid#206369PurposeExpresses mouse SOX2-C17 in mammalian cells, for lentivirus generation.DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR
Plasmid#174883PurposeTargeting vector for generating Pou5f1 STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Pou5f1
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-3XFLAG-AGO2
Plasmid#136687PurposeExpresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)DepositorInsertargonaute RISC Catalytic Component 2 (AGO2 Human)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SRRM2-mCherry
Plasmid#174089PurposeInducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on systemDepositorInsertSRRM2 (SRRM2 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterTREtightAvailable sinceSept. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir
Plasmid#90004PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]DepositorInsertsFKBP-linker-Myc-NBirA*
CBirA*-linker-FRB
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-CBir-FRB_Myc-NBir-FKBP
Plasmid#90003PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBPDepositorInsertsNBirA*-linker-FKBP
CBirA*-linker-FRB
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXG1
Plasmid#153124PurposeExpression of Flag tagged human FOXG1 in mammalian cellsDepositorInserthuman FOXG1 (FOXG1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP
Plasmid#90008PurposeSplit-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBPDepositorInsertsNBirA*-linker-FKBP
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir
Plasmid#90009PurposeSplit-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBPDepositorInsertsFKBP-linker-Myc-NBirA*
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas9(D10A)
Plasmid#74495PurposeNicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10ADepositorInsertCas9 D10A
UseSynthetic BiologyTagsExpressionBacterialMutationD10APromoterpTetoAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-CXXC(24)
Plasmid#124398PurposeFull-length TET1 missing zinc finger domain (585-624) used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 with CXXC zinc finger deletion (585-624) (TET1 Human)
UseTagsFlagExpressionMammalianMutationCXXC zinc finger deletion (aa 585-624)PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-tetpA-hNEUROG2-iRPT
Plasmid#140764PurposeExpresses iRFP713, PuroR and rtTAM2 in mammalian cells, with TRE-hNEUROG2DepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Y921F EphA2 pcDNA3
Plasmid#102736PurposeExpression of EphA2 in mammalian cellsDepositorInsertEphA2 (Epha2 Mouse)
UseTagsExpressionMammalianMutationY921F (TAC > TTC)PromoterCMVAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only