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Showing: 10101 - 10120 of 54324 results

  4. pET19-TdTc253

    Plasmid
    #207464
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 253
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    MTdTc253 (Dntt Mouse)
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Ser253Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…
    Promoter
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    207464-plasmid-map-sequence-id-407817

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  5. pET19-TdTc188

    Plasmid
    #207463
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 188
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    TdTc188 (Dntt Mouse)
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Cys216Ser, Cys302Ala, Cys378Ala, and Cys438Ser
    Promoter
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    207463-plasmid-map-sequence-id-407799

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  6. pET19-TdTc180

    Plasmid
    #207462
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 180
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    TdTc180 (Dntt Mouse)
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Glu180Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…
    Promoter
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    207462-plasmid-map-sequence-id-407791

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  7. pET19-TdTc302

    Plasmid
    #207461
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    TdTc302 (Dntt Mouse)
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser
    Promoter
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    207461-plasmid-map-sequence-id-407790

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  8. p Pcrg1: Khd4-Ada-Gfp (CbxR)

    Plasmid
    #203734
    Purpose
    Plasmid for ectopic integration and expression of Khd4-Ada-Gfp. The 4282 bp khd4-ORF is fused C-terminally to the 1200 bp adar catalytic domain (Ada) with three repeats of GGGGS linker in between, fol
    Depositor
    Michael Feldbrügge
    Article
    Sankaranarayanan et al Proc Natl Acad Sci U S A. 2023 Aug 22;12
    Insert
    Khd4-Ada-Gfp
    Use
    Tags
    Gfp
    Expression
    Bacterial
    Mutation
    Promoter
    Pcrg1
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    203734-plasmid-map-sequence-id-404932

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  9. pNMSB96

    Plasmid
    #199316
    Purpose
    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR at ttTi5605
    Depositor
    Michael Krieg
    Article
    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4
    Insert
    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    mTagBFP2 from pJJR81, C. elegans codon optimized …
    Promoter
    flp-18
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    199316-plasmid-map-sequence-id-404765

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  11. pET-Duet-6xHis-TEV-Nsp8(76-198)

    Plasmid
    #201024
    Purpose
    Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.
    Depositor
    Sharon Rozovsky
    Article
    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d
    Insert
    non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201024-plasmid-map-sequence-id-394447

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  20. Sniper2P

    Plasmid
    #193857
    Purpose
    Expresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/
    Insert
    Sniper2L
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    E1007L
    Promoter
    EFS
    Available since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    193857-plasmid-map-sequence-id-382456

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Showing: 10101 - 10120 of 54324 results