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Plasmid#68259PurposeLevel 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)DepositorInsertCoding sequence, hygromycin phophotransferase II (Escherichia coli)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNAflaB
Plasmid#149588Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmApple-GNG2
Plasmid#133857PurposeMammalian expression of GNG2 N-terminally tagged with mAppleDepositorInsertGNG2 (GNG2 Human)
UseTagsmAppleExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xFN-Tel-TAL/pCMV-7.1
Plasmid#63589PurposeExpresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat.DepositorInsert3xFVN-Tel-TAL
UseTALENTags3xFLAG-tag, V5-tag, and nuclear localization sign…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam N term myc eGFP 3C
Plasmid#160682PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneUseTagsmyc eGFP 3CExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-Squ:mCherry
Plasmid#20163DepositorInsertspaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR (sqh Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-ZapCY2
Plasmid#59016PurposeCytosolic zinc-binding FRET biosensorDepositorInsertNES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
UseTagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-SOMA
Plasmid#118935PurposeUsed to express the SOMA FRET sensor in E. coli.DepositorInsertSOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-mCherry
Plasmid#195161Purposemammalian expression of siRNA-resistant ABHD16A tagged with mCherryDepositorInsertABHD16A (ABHD16A Human)
UseTagsmCherryExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-SNAP
Plasmid#195164Purposemammalian expression of siRNA-resistant ABHD16A tagged with SNAP tagDepositorInsertABHD16A (ABHD16A Human)
UseTagsSNAP tagExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only