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Plasmid#75644Purpose3rd generation lentiviral gRNA plasmid targeting human CDK6DepositorInsertCDK6 (CDK6 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rix-PGK-Tom-W
Plasmid#25813DepositorInsertRix-PGK-Tom-W
UseLentiviralTagsExpressionMutationtdTomato from Tsien labPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSico-shNKCC1
Plasmid#83027Purposeexpresses an shRNA against NKCC1DepositorInsertshNKCC1
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα-RIIαWT-IRES-EGFP
Plasmid#183456PurposeReplacement of endogenous rat PKA-RIIα subunits with wild-type RIIαDepositorInsertsgRNA and Prkar2a (Prkar2a Rat)
UseLentiviralTagsExpressionMutationshRNA-resistant mutations: C135>G, G138>A, …PromotershRNA: H1 / gene: ubiquitinAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-TBCK_IDG-K
Plasmid#170587PurposeGateway destination clone of TBCK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-TBCK (TBCK Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.puro_shHuR 3UTR
Plasmid#110414PurposeTRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, puromycin selectionDepositorInsertELAVL1 (HuR)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA PolIII)Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
FH95pUGW (B3)
Plasmid#74012Purposelentiviral expression of EGFP and Psd95 shRNADepositorInsertPsd95
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
PIK3CA gRNA (BRDN0001145530)
Plasmid#77416Purpose3rd generation lentiviral gRNA plasmid targeting human PIK3CADepositorInsertPIK3CA (PIK3CA Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-2xAM_hIRF-1
Plasmid#92221PurposeLentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoterDepositorInsertsSp-dCas9-2xAM tag
gRNA targeting human IRF-1 promoter
UseCRISPR and LentiviralTags2xAM tagExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterCBh and U6Available sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
LATS1 gRNA (BRDN0001146581)
Plasmid#76024Purpose3rd generation lentiviral gRNA plasmid targeting human LATS1DepositorInsertLATS1 (LATS1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
LATS1 gRNA (BRDN0001146897)
Plasmid#76025Purpose3rd generation lentiviral gRNA plasmid targeting human LATS1DepositorInsertLATS1 (LATS1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) CRIP2
Plasmid#107509PurposeExpressing CRIP2, puromycin resistantDepositorInsertCRIP2 (codon optimized) (CRIP2 Human)
UseLentiviralTagsExpressionMammalianMutationCodon optimized sequencePromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a MS2-HP1cs 2A Hygro
Plasmid#102810PurposeLentiviral plasmid from FIRE-Cas9 system to express MS2-HP1cs fusion proteinDepositorInsertMS2-HP1cs
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Phage-PGK-GFP-T(WT)
Plasmid#181736Purposefor lentiviral expression of brachyury ORF in mammalian cellsDepositorInsertT (brachyury)
UseLentiviralTagsExpressionMutationa silent mutation in the PAM site of an sgRNAt ta…PromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Erh-V5
Plasmid#175123PurposeLentiviral expression of mouse Erh-V5DepositorInsertErh (Erh Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti
Plasmid#138318PurposeLenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFPDepositorInsertSauCas9 GFP sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
VRER variant
Plasmid#138561PurposeExpresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSDDepositorInsertVRER
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA4_MYOD1
Plasmid#64139PurposePhotoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA1_MYOD1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR sgSLC19A1
Plasmid#102314Purposegenetic depletion of SLC19A1DepositorInsertsgSLC19A1 (SLC19A1 Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_AAVS1B
Plasmid#183338PurposeAll-in-One CRISPRko system with a guide RNA that targets the AAVS1 locusDepositorInsertAAVS1B
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only