Addgene

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pRU48

Plasmid

#167670

Purpose

ENTRY vector for gRNA8 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aac

Plasmid

#129685

Purpose

(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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Wnt3A STOP

Plasmid

#35871

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR-Cdh1

Plasmid

#49776

Purpose

pENTR vector for mouse E-cadherin

Depositor

Jamie Davies

Article

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

Cdh1 (Cdh1 Mouse)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only
pEN_TmiRc3_shURB5

Plasmid

#81066

Purpose

Gateway entry vector encoding shRNA to UBR5

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

shRNA targeting UBR5

Use

Cloning

Tags

Expression

Mutation

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p5E-CMVSP6

Plasmid

#138356

Purpose

Gateway compatible 5' entry clone contains Cytomegalovirus and SP6 dual promoter(CMVSSP6)

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

CMV-SP6 promoter

Use

Gateway compatible

Tags

Expression

Mutation

Promoter

CMV-SP6

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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p221z-AtMIR390a

Plasmid

#118388

Purpose

Entry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

AtMIR390a backbone

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a - TR

Plasmid

#89621

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Thioredoxin

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ155

Plasmid

#69306

Purpose

Gateway entry vector with AteCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9D10A (Arabidopsis codon optimized)

Use

CRISPR

Tags

Expression

Mutation

D10A mutation; NLS fusion

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pEN_TmiRc3_shNT

Plasmid

#81067

Purpose

Gateway entry vector encoding non-targeting shRNA

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

non-targeting shRNA

Use

Cloning

Tags

Expression

Mutation

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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