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Showing: 981 - 1000 of 6476 results

  2. GST-CHMP4A (205-222) E209A (pGEX2T(TEV))

    Plasmid
    #80640
    Purpose
    Residues 205-222 of CHMP4A, Mutation modestly disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-206
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Changed Glutamate 209 to alanine
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80640-plasmid-map-sequence-id-161707

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  3. pFastBac HTB His-TEV-rDPP9(S729A)-FLAG

    Plasmid
    #217329
    Purpose
    Purification of catalytically-dead rat DPP9 fusion with C-terminal FLAG tagfrom insect cells
    Depositor
    Yifan Wang
    Insert
    DPP9
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    S729A
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217329-plasmid-map-sequence-id-433435

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  4. pFastBac HTB His-TEV-BN-rNLRP1(N61K)-FLAG

    Plasmid
    #217325
    Purpose
    Purification of Brown Norway rat NLRP1 (N61K mutation) fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    N61K
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217325-plasmid-map-sequence-id-438441

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  5. pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG

    Plasmid
    #217324
    Purpose
    Purification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    S969A
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217324-plasmid-map-sequence-id-438435

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  6. pFastBac HTB His-TEV-LEW-rNLRP1-FLAG

    Plasmid
    #217320
    Purpose
    Purification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217320-plasmid-map-sequence-id-438445

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  9. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII

    Plasmid
    #217897
    Purpose
    Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag
    Depositor
    Brenda Schulman
    Article
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    Use
    Tags
    2xSTII
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    217897-plasmid-map-sequence-id-432185

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  12. pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO

    Plasmid
    #199781
    Purpose
    Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).
    Depositor
    Sascha Martens
    Insert
    NEMO (IKBKG Human)
    Use
    Tags
    GST-Thrombin-TEV-EGFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    199781-plasmid-map-sequence-id-418258

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  14. pGEX2T-TEV CHMP1B 186-199 L188A/L192A

    Plasmid
    #108288
    Purpose
    Expresses residues 186-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-286
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108288-plasmid-map-sequence-id-245273

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  15. pGEX2T-TEV CHMP1B 186-199 L192A/L195A

    Plasmid
    #108290
    Purpose
    Expresses residues 186-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-287
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108290-plasmid-map-sequence-id-245275

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  18. pTK-EdnrbE2-AVI-Tfap2B-tev-FLAG-2A-Citrine

    Plasmid
    #127776
    Purpose
    Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Tfap2B in frame with an Avi tag at the N terminus and FLAG-tag and Citrine at the C-terminus
    Depositor
    Tatjana Sauka-Spengler
    Article
    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517
    Insert
    tfap2b (TFAP2B Chicken)
    Use
    Unspecified
    Tags
    Avi
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127776-plasmid-map-sequence-id-250021

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  19. GST-CHMP4A (205-222) L217A (pGEX2T(TEV))

    Plasmid
    #80638
    Purpose
    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-204
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Residues 205-222; Changed Leucine 217 to Alanine
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80638-plasmid-map-sequence-id-161738

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  20. GST-CHMP4A (205-222) W220A (pGEX2T(TEV))

    Plasmid
    #80637
    Purpose
    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-203
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Residues 205-222; Changed Trp 220 to Ala
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80637-plasmid-map-sequence-id-161733

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Showing: 981 - 1000 of 6476 results