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Plasmid#198668PurposeAn Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiaeDepositorInsertXI2_DW
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.H02L
Plasmid#198667PurposeAn Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiaeDepositorInsertXI2_UP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.H01L
Plasmid#198665PurposeAn Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiaeDepositorInsertX3_UP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1
Plasmid#181812PurposeExpresses human MUC2 D1, residues 21-389DepositorInsertMuc2 D1 (MUC2 Human)
UseTags6xHistadineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-mitoGFP
Plasmid#44385DepositorInsertmitoGFP (COX8A Human)
UseLentiviralTagsCox8 targeting sequence and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPathBrick
Plasmid#65006PurposeE. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricksDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive native promotersAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
p21 shRNA1
Plasmid#25868DepositorInsertp21 (Cdkn1a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCRISPReporter-mCherry
Plasmid#65008PurposeE. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterUser-definedAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluu
Plasmid#105832PurposeFast indicator for extracellular glutamate imagingDepositorInsertiGluSnFR S72T variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluu
Plasmid#106122PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR S72T variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-NT-3/D15A
Plasmid#73035PurposeExpress an NT-3-BDNF hybrid neurotrophic factor in mammalian cellsDepositorInsertNT-3/D15A (NTF3 Human)
UseLentiviralTagsExpressionMammalianMutationMutated Aspartic acid in position 15 of the matur…PromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
p21 shRNA2
Plasmid#25869DepositorInsertp21 (Cdkn1a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluf
Plasmid#105833PurposeFast indicator for extracellular glutamate imagingDepositorInsertiGluSnFR E25D variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluf
Plasmid#106121PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR E25D variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2C
Plasmid#225070PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2C)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGA-red-mini
Plasmid#196338PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-red-maxi
Plasmid#196337PurposeEasy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGA-blue
Plasmid#196336PurposeEasy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1 with cleavable His tag
Plasmid#181813PurposeExpresses human MUC2 D1 with cleavable His tag, residues 21-389DepositorInsertMuc2 D1 (MUC2 Human)
UseTags6xHistadine followed by TEV cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8m
Plasmid#162372PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8m
UseTags6xHisExpressionMammalianMutationA25G F286YPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only