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pCS5-3HA/p14ARF

Plasmid

#78764

Purpose

To overexpress p14ARF in Mammalian Cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

p14ARF (CDKN2A Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
pCS3-MT-BX/p14ARF

Plasmid

#78761

Purpose

To overexpress p14ARF in Mammalian Cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

ARF (CDKN2A Human)

Use

Tags

6xMYC

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
pMYC BRGI aa597-1407

Plasmid

#19146

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

human BRG1 aa 597-1407 (PCR product)

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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Chicken ArcLight Q175

Plasmid

#53566

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Chicken ArcLight-Q175

Use

Tags

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCS3-MT-BX/ARF1-64

Plasmid

#78762

Purpose

To overexpress ARF1-64 in Mammalian Cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

ARF (CDKN2A Human)

Use

Tags

6xMYC

Expression

Mammalian

Mutation

1-64

Promoter

CMV IE94

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
pCS3-MT-BX/ARF65-132

Plasmid

#78763

Purpose

To overexpress ARF65-132 in Mammalian Cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

ARF (CDKN2A Human)

Use

Tags

6xMYC

Expression

Mammalian

Mutation

65-132

Promoter

CMV IE94

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
pMYC BRGI aa1400-1648

Plasmid

#19145

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

human BRG1 aa 1400-1648 (PCR product)

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
EBFP2-UtrCH

Plasmid

#124656

Purpose

F-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).

Depositor

Cheng-han Yu

Article

Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

Insert

Utrophin (UTRN Human)

Use

Tags

EBFP2

Expression

Mammalian

Mutation

see depositor comments below

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Chicken ArcLight S174

Plasmid

#53567

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Chicken ArcLight-S174

Use

Tags

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Sec61β-Flag-APEX2-SOPP3

Plasmid

#229072

Purpose

Expresse APEX2-SOPP3 in ER in mammalian cell

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the ER (APEX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-APEX2-SOPP3-3xNLS

Plasmid

#229074

Purpose

expresses APEX2-SOPP3 in the mammalian nucleus

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the mammalian nucleus (APEX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
COX8mls-Flag-APEX2-SOPP3

Plasmid

#229075

Purpose

Expresses APEX2-SOPP3 in the mitochondrial matrix

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the mitochondrial matrix (APEX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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APEX2-SOPP3-TM

Plasmid

#229077

Purpose

Expresses APEX2-SOPP3 in the cell surface

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the cell surface (APEX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Human USP47

Plasmid

#217624

Purpose

Mammalian expression of USP47

Depositor

Ethan Lee

Article

Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

Insert

USP47 (USP47 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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Drosophila Usp46-V5

Plasmid

#217620

Purpose

Mammalian expression of V5 tagged Drosophila Usp46

Depositor

Ethan Lee

Article

Spencer et al Nat Commun. 2023 Oct 5;14(1):6174. doi:

Insert

USP46 (Usp12-46 Fly)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ACVR1-TASA-R206H

Plasmid

#206182

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S194A, and R206H

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Expression

Mutation

S190A, S194A, R206H

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TSAA-R206H

Plasmid

#206183

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S192A, S194A, and R206H

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Expression

Mutation

S192A, S194A, R206H

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ACVR1-VSAS-R206H

Plasmid

#206185

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S192A, and R206H

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Expression

Mutation

T189V, S192A, R206H

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ACVR1-VASS-R206H

Plasmid

#206186

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S190A, and R206H

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Expression

Mutation

T189V, S190A, R206H

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
3xEGFP-mMeikin(1-391, E156R, R159E) - hRad21(142-275) - mMeikin(387-434)

Plasmid

#174712

Purpose

Mammalian expression of mouse Meikin with wild-type Separase cleavage site eliminated and C-terminal Separase cleavage site inserted

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin (Meikin Mouse)

Use

Tags

3xEGFP

Expression

Mammalian

Mutation

mMeikin: 1-391, E156R, R159E; hRad21: 142-275; mM…

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge