ACVR1-VSAS-R206H
(Plasmid #206185)

• Purpose: in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S192A, and R206H
• Depositing Lab: Mary Mullins
• Publication: Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1002/jbmr.4869.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: in vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: activin A receptor type 1
• Alt name: ALK2
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• Mutation: T189V, S192A, R206H
• Entrez Gene: ACVR1 (a.k.a. ACTRI, ACVR1A, ACVRLK2, ALK2, FOP, SKR1, TSRI)
• Tag / Fusion Protein:
  • FLAG tag (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV Forward

Resource Information

• Supplemental Documents:
  • hACVR1_R206H_VSAS.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry