-
Plasmid#208078PurposeExpression of Alternative human Replication Protein A (RPA1,4,3) in E. coliDepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only
-
pcDNA3.1-CHRFAM7A
Plasmid#62284Purposemammalian expression of human duplicated Alpha7 (CHRFAM7A)DepositorInsertCHRFAM7A (CHRFAM7A Human)
UseTagsExpressionMammalianMutationpartial duplication of CHRNA7PromoterCMVAvailable sinceMarch 3, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pB-bsd-Flag-CNP
Plasmid#126608PurposeExpresses N-terminal Flag-tagged human CNPDepositorInsert2'3' cyclic nucleotide phosphodiesterase (CNP Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCAGAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDblet
Plasmid#8848DepositorInsertARS doublet
UseYeast cloning vectorTagsExpressionYeastMutationThis vector contains, between AatII sites, a dire…PromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHelA12
Plasmid#176493PurposeExpresses the N-terminal His6-tagged SARS-CoV helicase in E coli.DepositorInsertSARS-CoV NSP13 (ORF1ab SARS-CoV)
UseTagsHis6 and T7ExpressionBacterialMutationV570IPromoterT7Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFlare9A-Plekha5E10 WT
Plasmid#209576Purposeminigene construct for Plekha5 E10 alternative splicingDepositorInsertPlekha5 (Plekha5 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-nsp7
Plasmid#201025PurposeExpresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.DepositorInsertnon-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationPromoterCMV-tet-ONAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_6xH
Plasmid#186461PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
UseTags6xHis and MBPExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
SRC_HUMAN_D0
Plasmid#79700PurposeThis plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSRC (SRC Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
p6XHis_NLS-SaCas9
Plasmid#101086PurposeExpress SaCas9 in bacteria with a 6xHis tag for purificationDepositorInsertCRISPR-associated protein Cas9/Csn1
UseCRISPRTagsTrxA-6xHis-STagExpressionBacterialMutationPromoterT7lacAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT
Plasmid#163979PurposeMammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tagDepositorInsertNLRP1 (NLRP1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-His6MBP-A3A-Gly6His6
Plasmid#187822PurposeExpression human APOBEC3A in E.coli.DepositorInsertAPOBEC3A (APOBEC3A Human)
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationnonePromoterT7 promoter (on the backbone)Available sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
FAK2_HUMAN_D0
Plasmid#79747PurposeThis plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFAK2 (PTK2B Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNS20-SpCas9-SNAP
Plasmid#113717PurposeBacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
SLK_HUMAN_D0
Plasmid#79733PurposeThis plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSLK (SLK Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNS38-SadCas9-SNAP
Plasmid#113718PurposeBacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationD10A, N580APromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6A CARD8 CARD-FLAG WT
Plasmid#164020PurposeMammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tagDepositorInsertCARD8 (CARD8 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized
Plasmid#166834PurposeExpression and purification of NLRP1 (without the PYD and CARD) from mammalian cellsDepositorInsertNLRP1 (NLRP1 Human)
UseTagsTEV-GFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B CARD8 UPA-CARD-mCherry WT
Plasmid#164011PurposeMammalian expression vector encoding CARD8 UPA-CARD with a C-terminal mCherry tag for imagingDepositorInsertCARD8 (CARD8 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
CDK2_HUMAN_D0
Plasmid#79726PurposeThis plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertCDK2 (CDK2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only