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pCellFree_G03 TFEB
Plasmid#67097PurposeStandard for cell-free expression benchmarking.DepositorInsertTFEB (TFEB Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a)TetR
Plasmid#172352PurposeTet Repressor reporterDepositorInsertsTetR
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
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P2A-NLS-tdtomato-EPG backbone
Plasmid#215548PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFPDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II-dCas13-APO1-YTH
Plasmid#204767PurposeGEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)DepositorInsertdCas13-NLS-APO1-YTH-HA
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEf1alphaAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAX198
Plasmid#173042PurposeCustom vector for sgRNA library constructionDepositorInsertHBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pINT-PuroDDHNF4a2
Plasmid#31443DepositorInsertHNF4A (HNF4A Human)
UsePhic31/attbTagsFKBP L106PExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
SOX17-NLS-tdTomato-EPG
Plasmid#210466PurposeDonor plasmid for knock-in NLS-tdTomato into the human SOX17 locusDepositorInsertSOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived puromycin-EGFP selection cassette (SOX17 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBA904
Plasmid#122238PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCM-CLYBL-NGN2-BRN3A
Plasmid#165597PurposeDonor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.DepositorInsertsUseCRISPR and TALENTagsExpressionMammalianMutationPromoterCAG, EF-1alpha, Endogenous CLYBL (splice acceptor…Available sinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMS1431
Plasmid#29226PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle77 (GABRA6 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1434
Plasmid#29229PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle80 (GABRA6 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1058
Plasmid#29008PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle78 (GABRA6 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsCre-EGFP-NLSExpressionMutationPromoterAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only
