Addgene

We narrowed to 2,851 results for: multiplex

Showing: 981 - 1000 of 2851 results

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pEE388

Plasmid

#149286

Purpose

T-DNA encoding TRV2 with mFT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1_mFT:tNos

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only
pU6_(GLuc)_sgRNA

Plasmid

#68422

Purpose

Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Gluc sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNeo3/Cre

Plasmid

#89653

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ158

Plasmid

#69307

Purpose

Gateway entry vector with hSpCas9 (Human codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

hSpCas9 (Human codon optimized)

Use

CRISPR

Tags

3XFLAG

Expression

Mutation

NLS fusion

Promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pFUS_A8

Plasmid

#99882

Purpose

Recipient vector for Golden Gate TALE cloning system. Used to assemble 8 TALE repeats.

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS3-IVS-Syn21-GFP-P10

Plasmid

#104614

Purpose

Expresses GFP upon binding of TALE3 to VAS3

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS3

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS1-IVS-Syn21-GFP-P10

Plasmid

#104613

Purpose

Expresses GFP upon binding of TALE1 to VAS1

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS1

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ265_BasA3G

Plasmid

#225145

Purpose

Gateway entry clone (attL1 & attR5) BasA3G-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Inserts

BasA3G-zCas9(D10A)-UGI
-

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ265_EtA3C

Plasmid

#225146

Purpose

Gateway entry clone (attL1 & attR5) EtA3C-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

EtA3C-zCas9(D10A)-UGI

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ265_OoA3GX2

Plasmid

#225147

Purpose

Gateway entry clone (attL1 & attR5) OoA3GX2-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

OoA3GX2-zCas9(D10A)-UGI

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ265_DlA3G

Plasmid

#225148

Purpose

Gateway entry clone (attL1 & attR5) DlA3G-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

DlA3G-zCas9(D10A)-UGI

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ265_LoA3GX1

Plasmid

#225149

Purpose

Gateway entry clone (attL1 & attR5) LoA3GX1-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

LoA3GX1-zCas9(D10A)-UGI

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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act5C-mScarlet

Plasmid

#230906

Purpose

Ubiquitous expression of mScarlet in Drosophila melanogaster

Depositor

Michael Boutros

Article

Port et al bioRxiv 2024.11.26.625385

Insert

mScarlet

Use

Tags

Expression

Insect

Mutation

Promoter

act5C

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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UAST-uMCas12a+nls2x

Plasmid

#230909

Purpose

inducible expression of Cas12a+ with Gal4.

Depositor

Michael Boutros

Article

Port et al bioRxiv 2024.11.26.625385

Insert

Cas12a+

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGRACE

Plasmid

#230910

Purpose

Tracing of CRISPR activity in whole animals.

Depositor

Michael Boutros

Article

Port et al bioRxiv 2024.11.26.625385

Insert

GFP

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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LAG3-DEM (L3#1)

Plasmid

#227739

Purpose

Designer Epigenome Modifier (DEM) designed to target the LAG3-promoter

Depositor

Claudio Mussolino

Article

Azcona et al Methods Mol Biol. 2024;2842:209-223. doi

Insert

Designer epigenome modifier targeting LAG3-promoter (Homo sapiens)

Use

TALEN

Tags

DNMT3A, DNMT3L, KRAB

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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apt-CBE-SL3

Plasmid

#219940

Purpose

Mammalian expression of apt-CBE-SL3 protein (MCP_16aa-linker_evoAPOBEC1) with P2A-EGFP marker

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

MCP_16-aa_evo-rA1_2xUGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST P38KTRDronpa

Plasmid

#205763

Purpose

Expression of P38 KTR Dronpa under CMV promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

P38 KTR Dronpa

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST ERKKTRrsFastLime

Plasmid

#205765

Purpose

Expression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

ERK KTR rsFastLime

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCF.111

Plasmid

#227187

Purpose

pORTMAGE-Ec1 + supplement E. coli SSB

Depositor

Seth Shipman

Article

Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

Insert

CspRecT, mutL_E32K, E. coli SSB

Use

Tags

Expression

Bacterial

Mutation

mutL E32K

Promoter

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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