Addgene

Showing: 981 - 1000 of 10154 results

Results per page:

AP334-6

Plasmid

#66097

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs5

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only
APCSD 53

Plasmid

#66099

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for CSD 53

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only
APCSD 54

Plasmid

#66100

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for CSD 54

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only
pTRIPZ-hDDX5/17

Plasmid

#71307

Purpose

Inducible shRNA knockdown of both human DDX5 and DDX17 genes

Depositor

Allan Brasier

Article

Yang et al Mol Cell Proteomics. 2015 Oct;14(10):270

Insert

DDX5/17 shRNA (DDX17 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Availability

Academic Institutions and Nonprofits only

Enlarge
pCASCADE-gltA1-gltA2

Plasmid

#71348

Purpose

gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

gltA1-gltA2 guide array

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Availability

Academic Institutions and Nonprofits only

Enlarge
pCoHygro L16_shRNA of spliced Copia

Plasmid

#71389

Purpose

knock down expression of spliced Copia in Drosophila melanogaster

Depositor

Timothy Nilsen

Insert

Copia

Use

Tags

Expression

Insect

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pRB1081

Plasmid

#71479

Purpose

Expresses gRNA for dpy-10 conversion using NGAG PAM and VQR Cas9 variant

Depositor

Andrew Fire

Article

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

dpy-10 gRNA

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
sgPal1 (p2Tol-U6-sgPal1-HygR)

Plasmid

#71483

Purpose

Expresses palindromic sgRNA for use in self-cloning CRISPR

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Insert

sgPal1

Use

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
sgPal7 (p2Tol-U6-sgPal7-HygR)

Plasmid

#71484

Purpose

Expresses palindromic sgRNA for use in self-cloning CRISPR

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Insert

sgPal7

Use

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pRB1084

Plasmid

#71516

Purpose

Expresses gRNA for dpy-10 conversion using NGCG PAM and VRER Cas9 mutant

Depositor

Andrew Fire

Article

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

dpy-10 gRNA

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
SS9_RNA

Plasmid

#71656

Purpose

SS9-targeting gRNA for co-transformation with pSS9

Depositor

Ryan Gill

Article

Bassalo et al ACS Synth Biol. 2016 Apr 22.

Insert

SS9 gRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Availability

Academic Institutions and Nonprofits only

Enlarge
pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

Plasmid

#71828

Purpose

Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control

Depositor

Vlatka Zoldoš

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

BACH2-sgRNA8 (BACH2 S. pyogenes, Human, Synthetic)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

Plasmid

#71829

Purpose

Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control

Depositor

Vlatka Zoldoš

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

BACH2-sgRNA8 (BACH2 S. pyogenes, Human, Synthetic)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9; E756A inactiva…

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pdCas9-DNMT3A-PuroR_hNT

Plasmid

#71830

Purpose

Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells

Depositor

Vlatka Zoldoš

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

Non-targeting sgRNA human (DNMT3A S. pyogenes, Human, Synthetic)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIR-21 Reporter

Plasmid

#71870

Purpose

Mammalian expression vector for the analysis of miR-21 activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Reverse complementary sequence of miR-21

Use

Tags

firefly luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIR-31-Reporter

Plasmid

#71871

Purpose

Mammalian expression vector for the analysis of miR-31 activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Reverse complementary sequence of miR-31

Use

Tags

firefly luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIR-192-Reporter

Plasmid

#71872

Purpose

Mammalian expression vector for the analysis of miR-19α activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Reverse complementary sequence of miR-192

Use

Tags

firefly luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pMIR-193a-5p-Reporter

Plasmid

#71873

Purpose

Mammalian expression vector for the analysis of miR-193a-5p activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Reverse complementary sequence of miR-193a-5p

Use

Tags

firefly luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pMIR-31-192-193a-Reporter

Plasmid

#71874

Purpose

Mammalian expression vector for the analysis of miR-31-19α-193a activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Binding sequence targeted by miR-31, miR192, and miR-193a-5p

Use

Tags

firefly luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
Lenti-sgLkb1/Cre

Plasmid

#66894

Purpose

Expresses an Lkb1-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

Insert

sgLkb1

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge