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Rab8WT(Flag) in pcDNA3.1neo

Plasmid

#46783

Purpose

expression of WT Rab8a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB8A (RAB8A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pEF274 SVAfw

Plasmid

#178192

Purpose

The SVA (SINE-VNTR-ALU) retroposon that causes human X-linked Dystonia Parkinsonism (XDP), within a BAC (pJazz) backbone.

Depositor

Elizabeth Fisher

Insert

SVA that inserts into the X-linked human TAF1 gene to cause X-linked dystonia Parkinsonism

Use

Linear bac vector

Tags

Expression

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pIDS-LSD1fl

Plasmid

#109157

Purpose

Encodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length LSD1, sequence-optimized for insect cells (KDM1A Human)

Use

Tags

Expression

Insect

Mutation

Promoter

p10

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pACAGW-ChR2-Venus-AAV

Plasmid

#20071

Purpose

AAV-mediated expression of ChR2-Venus for neuronal excitation

Depositor

Karel Svoboda

Article

Leopoldo et al Nature. 2009 Jan 18.

Has Service

AAV1 and AAV9

Insert

Channelrhodopsin-2 (CHLRE_02g085257v5 Chlamydomonas reinhardtii)

Use

AAV

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CAGG

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pBS hTSP-4

Plasmid

#13001

Depositor

Jack Lawler

Article

Lawler et al J Biol Chem. 1995 Feb 10. 270(6):2809-14

Insert

TSP-4 (THBS4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-mRuby2

Plasmid

#99123

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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NylB'-SCY_pET21b

Plasmid

#215417

Purpose

Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB'-SCY

Use

Tags

Expression

Bacterial

Mutation

R187S/F264C/D370Y

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-BMAL1

Plasmid

#186829

Purpose

Expresses FLAG-BMAL1 in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJ3 SHP2 WT

Plasmid

#8317

Depositor

Ben Neel

Article

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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pCI-neo Flag HAUSP

Plasmid

#16655

Depositor

Bert Vogelstein

Article

Cummins et al Cell Cycle. 2004 Jun . 3(6):689-92.

Insert

HAUSP (USP7 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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