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Plasmid#85114Purposetransfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusionDepositorInserthuman full-length METTL20 (METTL20 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGT8162 (AATG_dttLbCas12a-i_TTCG)
Plasmid#192350PurposeL0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expressionDepositorInsertAATG_dttLbCas12a-i_TTCG
UseCRISPRTagsExpressionMutationD156R; E925QPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sm-Myocardin Omni-pShuttle
Plasmid#122122PurposeExpression of the smooth muscle version of myocardinDepositorInsertMyocardin (Myocd Mouse)
UseTagsOmniExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV-EGFP-C
Plasmid#13887DepositorInsertC domain of N-WASP (WASL Bovine)
UseAdenoviralTagsEGFPExpressionMutationThe BglII site in the MCS of pShuttle-CMV was fil…PromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
Puro-TTL-wt-TTF-1
Plasmid#67657PurposeTo create a dox-on inducible expression of human wt-TTF-1 cDNA in cellsDepositorInsertThyroid Transcription Factor 1 (NKX2-1 Human)
UseRetroviralTagsN/AExpressionMutationPromoterAvailable sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
px335 Mettl14 sgRNA #1
Plasmid#61515Purposeencodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)DepositorInsertgcggcagctcctagctcagc
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-METTL14-WT
Plasmid#141118PurposeRetroviral overexpression of WT METTL14 in mammalian cells.DepositorInsertMETTL14 (METTL14 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL1-TTlight-4xDQNAT
Plasmid#128394PurposeIn vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sitesDepositorInsertTetanus toxin light chain domain
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationInactivating E234A mutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK TTLL11 3'UTR
Plasmid#19857DepositorInsertTTLL11 3'UTR (TTLL11 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pShuttle AMPK gamma1
Plasmid#15998DepositorInsertAMPK gamma1 (Prkag1 Mouse)
UseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLYS6-METTL17-FLAG
Plasmid#220875PurposeExpressing METTL17-FLAGDepositorInsertMETTL17 (METTL17 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-FLAG-HA-mmuMettl3
Plasmid#207122PurposeLentiviral plasmid to express mouse Mettl3DepositorInsertMettl3 (Mettl3 Mouse)
UseLentiviralTagsFLAG-HAExpressionMutationPromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-FLAG-HA-mmuMettl3-LN
Plasmid#207129PurposeLentiviral plasmid to express mouse Mettl3 Lambda peptide C-terDepositorInsertMettl3 (Mettl3 Mouse)
UseLentiviralTagsFLAG-HA and Lambda Peptide (LN)ExpressionMutationPromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FLAG-METTL3-APPA
Plasmid#160251PurposeExpress catalytic inactive FLAG-METTL3 in human cellsDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationchanges in aa 395–398, DPPW → APPAPromoterCMVAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV-F2A-T2A
Plasmid#62622PurposeAdEasy Shuttle vector modified for multicistronic expression. Contains T2A and F2ADepositorInsertMCS with F2A and T2A
UseAdenoviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-dCasRx-METTL3-PURO
Plasmid#174218PurposeExpresses dCasRx-METTL3 in mammalian cellDepositorInsertdCasRx-METTL3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterpMSCVAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR3
Plasmid#213051PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pShuttle CMV RGECO-TnT
Plasmid#124643PurposeMyofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination.DepositorInsertRGECO-TnT (TNNT2 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-mRFP-gsk3 s9a
Plasmid#24371DepositorInsertGSK3beta(S9A) (GSK3B Human)
UseAdenoviralTagsmRFPExpressionMammalianMutationS9A. Mutant version of GSK3 that lacks the inhibi…PromoterAvailable sinceMay 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR5
Plasmid#213053PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only