Addgene

We narrowed to 208 results for: prion

Showing: 81 - 100 of 208 results

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pRS306Sup35R1+7

Plasmid

#17720

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+7 (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pRS306Sup35R1+8

Plasmid

#17721

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+8 (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pRS306Sup35R1+8H1Q

Plasmid

#17722

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+8H1Q (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pGAN230

Plasmid

#131122

Purpose

reverse yTRAP, RNQ1-aTF reporting on tetR-mKate2

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

SUP35

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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p416TEF-NYFP

Plasmid

#99553

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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p416TEF-NQYFP

Plasmid

#99552

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 GPD-Sis1deltaG/F

Plasmid

#18688

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Sis1 deltaG/F

Use

Tags

Expression

Yeast

Mutation

Sis1 with the G/F region deleted

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pWJPrP5

Plasmid

#22106

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

mouse PrP ORF insert (Prnp Mouse)

Use

Construction of targeting vector

Tags

Expression

Mutation

mutated codon 177 (D to N)

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
p415TEF-NQYFP

Plasmid

#99554

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pX335_HR_Prnp_3

Plasmid

#78622

Purpose

pX335 vector encoding SpCas9n and a chimeric guide RNA targeting Prnp coding sequence

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

HR_Prnp_3 (Prnp Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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sgRNA(MS2)_Prnp_SAM2

Plasmid

#78625

Purpose

Encodes sgRNA2.0 targeting 138nt upstream of TSS

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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sgRNA(MS2)_Prnp_SAM1

Plasmid

#78624

Purpose

Encodes sgRNA2.0 targeting 158nt upstream of TSS

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pJC25R1+8stop

Plasmid

#17724

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+8 (SUP35 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pJC25R1+8H1Qstop

Plasmid

#17725

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+4 (SUP35 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys

Plasmid

#15602

Depositor

Susan Lindquist

Article

Tessier et al Nature. 2007 May 31. 447(7144):556-61.

Insert

Sup35 NM (SUP35 Candida albicans, Budding Yeast)

Use

Tags

7xHis and Cysteine

Expression

Bacterial

Mutation

4 G/A. Four glycines mutated to alanines. The pos…

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pJCNM(del22-68)

Plasmid

#1095

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Use

Tags

His

Expression

Bacterial

Mutation

amino acids 20 - 68 DELETED from NM domain of SUP…

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
pJLI-Sup35N-KDG6-C

Plasmid

#1238

Depositor

Susan Lindquist

Article

Liu et al Proc Natl Acad Sci U S A 2002 Dec 10;99

Insert

SUP35 (SUP35 Budding Yeast)

Use

Yeast integrative plasmid

Tags

Expression

Mutation

SUP35 middle region (aa124-253) was replaced with…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pSK221

Plasmid

#129249

Purpose

reverse yTRAP, tetR-responsive Neon reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

pADH1(tetO2)-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGAN147

Plasmid

#129202

Purpose

yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

SUP35

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
cytosolic moPrP (3F4)

Plasmid

#1321

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP (Prnp Mouse)

Use

Tags

Expression

Mammalian

Mutation

N-term truncation (to make gene cytosolic), vario…

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only