Addgene

We narrowed to 526 results for: prime editing

Showing: 81 - 100 of 526 results

Results per page:

Sort results by:

p2T_CAG_SpCas9PE2_5plinker_BlastR

Plasmid

#173066

Purpose

Expresses PE2

Depositor

Richard Sherwood

Article

Velimirovic et al Nat Commun. 2022 Jun 18;13(1):3512. doi:

Insert

5plinker

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPBT-PE2-PuroTK-pegRNA_GG

Plasmid

#173222

Purpose

piggyBac transposon containing both PE2 and a pegRNA cloning site.

Depositor

Jacob Giehm Mikkelsen

Article

Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

Inserts

PE2
PuroTK
pegRNA cloning cassette

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

CMV, P2A, and hU6

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-CMP-PE-V1

Plasmid

#202769

Purpose

Expresses CMP-PE-V1 in mammalian cells for prime editing

Depositor

Kyoungmi Kim

Article

Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

Insert

CMP-PE-V1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-NRCH-PE2-BSD

Plasmid

#191101

Purpose

Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

NRCH-PE2-BSD

Use

CRISPR and Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiGuide-BlastR

Plasmid

#196970

Purpose

Lentiviral acceptor vector for pegRNAs with blasticidin resistance

Depositor

Leopold Parts

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PE2max-BSD

Plasmid

#191102

Purpose

Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

PEmax-P2A-BSD

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Changed R221K, N394K, H840A from SpCas9

Promoter

EF-1a

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PE-Plus

Plasmid

#214079

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

PE-Plus

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
rnLbCas12a-PE2

Plasmid

#213746

Purpose

For circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

rnLbCas12a-M-MLV RT∆RNase H

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R, H759A, R1138A in LbCas12a

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-NRCH-PE2max-BSD

Plasmid

#191103

Purpose

Lentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

NRCH-PEmax

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Changed R221K, N394K, H840A from SpCas9-NRCH

Promoter

EF-1a

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PE2-BSD

Plasmid

#161514

Purpose

Expression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selection

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Sep 21. pii: 10.103

Insert

PE2-P2A-BSD

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF-1α core promoter

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PE2-tagRFP-BleoR

Plasmid

#192508

Purpose

Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.

Depositor

Gerald Schwank

Article

Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

PE2-P2A-tagRFP-P2A-BleoR

Use

Tags

BleoR, SV40 bpNLS, and tagRFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-CMV-PEmax-IRES-ZeoR

Plasmid

#207353

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

Prime editor max (PEmax) enzyme

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-CMV-PE2-IRES-ZeoR

Plasmid

#207352

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

Prime editor 2 (PE2) enzyme

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PEmax-P2A-GFP

Plasmid

#180020

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-EGFP

Use

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PEmax-P2A-hMLH1dn

Plasmid

#174828

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-hMLH1dn

Use

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript; deletion of residues 754-…

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PE2-P2A-hMLH1dn

Plasmid

#174827

Purpose

Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PE2-P2A-hMLH1dn

Use

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript; deletion of residues 754-…

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PEmax-P2A-BSD

Plasmid

#174821

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-BSD

Use

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-tevopreq1-GG-acceptor

Plasmid

#174038

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-tmpknot-GG-acceptor

Plasmid

#174039

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T_CAG_SpCas9PE2_Igf1-NFATC2IP_BlastR (pepPE2_3)

Plasmid

#173067

Purpose

Expresses NI-PE2

Depositor

Richard Sherwood

Article

Velimirovic et al Nat Commun. 2022 Jun 18;13(1):3512. doi:

Insert

Igf1-NFATC2IP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge