Addgene

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pEF Synaptophysin-GCamp6s

Plasmid

#188981

Purpose

Presynaptic fluorescent Ca2+ indicator

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Synaptophysin (Syp Rat, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pJFRC7-syt1-GCaMP6f

Plasmid

#190896

Purpose

GCaMP6f fused to Drosophila synaptotagmin1 via a 3x GS linker. In the pJFRC7-20XUAS vector.

Depositor

Tom Clandinin

Article

Turner et al Elife. 2022 Oct 27;11. pii: 82587. doi:

Insert

syt1-GCaMP6f

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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CMV-mt4xi-GCaMP6f

Plasmid

#192075

Purpose

Fluorescent reporter for mitochondrial calcium

Depositor

Timothy Ryan

Insert

4 repeats of improved COX8 signal peptide plus GCaMP6f

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-GCaMP6f

Plasmid

#135632

Purpose

AAV vector to drive the expression of GCaMP6f in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mutation

None

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-S1-GCaMP6f

Plasmid

#160722

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S1-GCaMP6f

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Availability

Academic Institutions and Nonprofits only

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pKL11-GCaMP6f bb100

Plasmid

#158983

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

Depositor

Joel Kralj

Article

Bruni et al Elife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f

Use

Tags

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23100 (sigma 70)

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomaGCaMP6f1

Plasmid

#158755

Purpose

Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP6f1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

synapsin promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomaGCaMP6f2

Plasmid

#158756

Purpose

Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP6f2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

synapsin promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SomaGCaMP6f2

Plasmid

#158757

Purpose

Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Has Service

AAV8 and AAV9

Insert

SomaGCaMP6f2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-OTp-GCaMP6s

Plasmid

#192945

Purpose

To drive GCaMP6s under the control of mouse oxytocin promoter

Depositor

Kazunari Miyamichi

Article

Yaguchi et al PLoS One. 2023 May 10;18(5):e0285589. do

Insert

GCaMP6s

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Hygro_GCaMP6s

Plasmid

#164591

Purpose

Stable integration and expression of GCaMP6s in mammalian cells

Depositor

Joseph Hyser

Article

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

GCaMP6s

Use

Lentiviral

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCS2+ GCamp6-3NLS

Plasmid

#165401

Purpose

Expresses a nuclear localized genetically encoded calcium reporter.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

Nuclear Localization Signal

Use

Avian, xenopus, zebrafish

Tags

GCamp6

Expression

Mammalian

Mutation

Promoter

SP6

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GCaMP6f

Plasmid

#166022

Purpose

Expresses GCaMP6f in CaMKII+ cells

Depositor

Xue Han

Article

Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588.

Insert

GCaMP6f

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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slc1a3b:myrGCaMP6s-P2A-H2AmCherry

Plasmid

#170209

Purpose

Drives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleus

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

slc1a3b (slc1a3b Zebrafish)

Use

Zebrafish

Tags

myrGCaMP6s-P2A-H2AmCherry

Expression

Mutation

Promoter

slc1a3b

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD4-GCaMP6f

Plasmid

#178727

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-VTKD5-GCaMP6f

Plasmid

#178728

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-VTKD6-GCaMP6f

Plasmid

#178729

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-VTKS4-GCaMP6f

Plasmid

#178730

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-VTKS5-GCaMP6f

Plasmid

#178731

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-VTKS6-GCaMP6f

Plasmid

#178732

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge